GRB10 Antibody - BSA Free

Immunohistochemistry: GRB10 Antibody [NB100-88153] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-GRB10 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Immunohistochemistry-Paraffin: GRB10 Antibody [NB100-88153] - FFPE section of human ovarian carcinoma. Affinity purified rabbit anti-GRB10 used at a dilution of 1:250

Immunohistochemistry-Paraffin: GRB10 Antibody [NB100-88153] - Human colon carcinoma. Antibody used at a dilution of 1:1,000.

Immunoprecipitation: GRB10 Antibody [NB100-88153] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-88153 used for IP at 3 mcg/mg lysate. GRB10 was also immunoprecipitated by rabbit anti- GRB10 antibody NB100-88152, which recognizes an upstream epitope.

• Reactivity: Hu
• Applications: IHC, IP, WB (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

GRB10 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit GRB10 Antibody - BSA Free (NB100-88153) is a polyclonal antibody validated for use in IHC and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 100 and 150 of human growth factor receptor-bound protein 10 using the numbering given in entry NP_005302.3 (GeneID 2887).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GRB10
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:250
  • Immunoprecipitation 2-5 ug/mg lysate
• Theoretical MW: 67 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GRB10 Antibody - BSA Free

• GRB10 adapter protein
• GRB10 adaptor protein
• Grb-10
• GRBIR
• GRB-IR
• growth factor receptor-bound protein 10
• Insulin receptor-binding protein Grb-IR
• IRBP
• KIAA0207
• maternally expressed gene 1
• MEG1
• RSS

Background

The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors. Overexpression of some isoforms of the encoded protein inhibits tyrosine kinase activity and results in growth suppression. This gene is imprinted in a highly isoform- and tissue-specific manner. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GRB10
• Entrez:
  • 2887(Human)
• Uniprot:
  • Q13322(Human)