Rad50 Antibody


Western Blot: RAD50 Antibody [NBP2-20055] - Sample (30 ug of whole cell lysate) A: NT2D1 5% SDS PAGE gel, diluted at 1:5000.
Immunohistochemistry-Paraffin: RAD50 Antibody [NBP2-20055] - Immunohistochemical analysis of paraffin-embedded Lung carcinoma, using antibody at 1:250 dilution.
Western Blot: RAD50 Antibody [NBP2-20055] - Rad50 antibody [N1N2], N-term detects RAD50 protein by Western blot analysis. A. 30 ug Neuro2A whole lysate/extract B. 30 ug GL261 whole cell lysate/extract5 % SDS-PAGERad50 ...read more

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P
0.78 mg/ml

Order Details

Rad50 Antibody Summary

Recombinant fragment corresponding to a region within amino acids 128 and 420 of Rad50 (Uniprot ID#Q92878)
Nucleus; Telomere
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7).
0.01% Thimerosal
0.78 mg/ml
Immunogen affinity purified


Application Notes
suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
154 kDa

Reactivity Notes

Human, Mouse.

Alternate Names for Rad50 Antibody

  • DNA repair protein RAD50
  • EC 3.6
  • EC
  • EC 3.6.3
  • EC
  • hRad50
  • RAD50 (S. cerevisiae) homolog
  • RAD50 homolog (S. cerevisiae)
  • RAD502
  • RAD50-2


The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. [provided by RefSeq]


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies


Isotype Controls

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Blogs on Rad50.

RAD50 and DNA Damage Response
DNA repair protein RAD50 is a component of the MRN complex (Mre11-RAD50-Nbs1) responsible for DNA double strand break (DSB) repair. DSBs are caused by ionizing radiation, certain chemotherapy drugs, metabolic reactive oxygen species (ROS), replication...  Read full blog post.

The MRE11 Complex and DNA Damage Response
The maintenance of genome stability depends on the DNA damage response (DDR) which is a complex signaling network including cell cycle checkpoints, DNA repair and damage tolerance pathways. The DDR complex has the ability to sense DNA damage and tra...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol RAD50

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