Rad50 Antibody - Azide and BSA Free

Western Blot: Rad50 Antibody [NB100-1487] - Detection of Rad50 using NB100-1487. Lane 1: 25 ng purified hMre11-hRad50 complex Lane 2: 25 ug crude HeLa extract.

Simple Western: Rad50 Antibody [NB100-1487] - Simple Western lane view shows a specific band for Rad50 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Rad50 Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit Rad50 Antibody - Azide and BSA Free (NB100-1487) is a polyclonal antibody validated for use in WB, Simple Western and IP. Anti-Rad50 Antibody: Cited in 5 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human RAD50 zinc hook region (amino acids 628-787) expressed in E. coli. [UniProt# Q92878]
• Localization: Upon DNA damage, Rad50 and Mre11 redistribute and are co-localized within the nucleus of damaged cells.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RAD50
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1-5ul
  • Simple Western 1:1000
  • Western Blot 1:1000-1:10000
• Application Notes: This RAD50 antibody is useful for Immunoprecipitation and Western blot, where a band can be seen at approximately 153 kDa.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in HeLa lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:1000, apparent MW was 149 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 153 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: No Preservative
• Purity: Unpurified

Alternate Names for Rad50 Antibody - Azide and BSA Free

• DNA repair protein RAD50
• EC 3.6
• EC 3.6.1.15
• EC 3.6.3
• EC 3.6.3.27
• hRad50
• NBSLD
• RAD50 (S. cerevisiae) homolog
• RAD50 homolog (S. cerevisiae)
• Rad50
• RAD502
• RAD50-2

Background

RAD50 (DNA repair protein RAD50) is a component of MRN complex (Mre11-Rad50-Nbs1) that plays an important role in detection and signaling of DNA double strand breaks (DSBs) through acting as DSB sensor, co-activator of DSB-induced cell cycle checkpoint signaling, and as repair-effector in two competing DSB repair pathways: homologous recombination (HR) and non-homologous end-joining (NHEJ). MRN complex also associates with telomeres at the ends of linear chromosomes, where it contributes to their maintenance. RAD50 is required to bind DNA ends for holding them in close proximity where it facilitates searches for short or long regions of sequence homology in the recombining DNA templates, and also stimulate the activity of DNA ligases and/or restrict MRE11A's nuclease activity to prevent nucleolytic degradation. DSBs can be caused by ionizing radiation, certain chemotherapy drugs, metabolic ROS, as errors during replication, by programmed enzymatic activities during meiosis/V(D)J recombination etc., and if left unrepaired, DSBs can generate chromosomal translocations, aneuploidy and carcinogenesis. Defects in RAD50 have been linked to Nijmegen breakage syndrome-like disorder (NBSLD).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Rad50 Antibody (NB100-1487)(5)

Publications using NB100-1487

• Patil S, Chalkiadaki K, Mergiya TF, Krimbacher K et Al. eIF4E phosphorylation recruits ?-catenin to mRNA cap and promotes Wnt pathway translation in dentate gyrus LTP maintenance iScience 2023-05-30 [PMID: 37250335]
• Zhou H, Kawamura K, Yanagihara H et al. NBS1 is regulated by two kind of mechanisms: ATM-dependent complex formation with MRE11 and RAD50, and cell cycle-dependent degradation of protein J Radiat Res. 2017-03-22 [PMID: 28369484] (WB, Human)
  
  Details:
  The regulatory mechanisms of the MRN complex, formed by NBS1 with MRE11 and RAD50, are investigated through immunoprecipitation.
• Chang L, Huang J, Wang K et al. Targeting Rad50 sensitizes human nasopharyngeal carcinoma cells to radiotherapy. BMC Cancer 2016-03-07 [PMID: 26951044] (WB)
• Kobayashi J, Saito Y, Okui M, Miwa N. Increased oxidative stress in AOA3 cells disturbs ATM-dependent DNA damage responses Mutation Research/Genetic Toxicology and Environmental Mutagenesis 2015-03-18 [PMID: 25868131] (WB, Human)
• Cahill, D et al. Dimerization of the Rad50 protein is independent of the conserved hook domain. Mutagenesis;22(4):269-74. 2007-07-01 [PMID: 17426050] (WB, Human)

Bioinformatics

• Gene Symbol: RAD50
• Entrez:
  • 10111(Human)
• Uniprot:
  • Q92878()