Rad17 Overexpression Lysate (Native)

Images

 
Western Blot: Rad17 Overexpression Lysate (Adult Normal) [NBL1-15112] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Rad17.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Rad17 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC207114 and based on accession number NM_002873. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens RAD17 homolog (S. pombe) (RAD17), transcript variant 8, mRNA.
Gene
RAD17

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
75.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Rad17

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Rad17 Overexpression Lysate (Native)

  • CCYC
  • cell cycle checkpoint protein (RAD17)
  • cell cycle checkpoint protein RAD17
  • FLJ41520
  • HRAD17
  • R24L
  • RAD1 (S. pombe) homolog
  • RAD1 homolog
  • RAD17 homolog (S. pombe)
  • Rad17
  • Rad17-like protein
  • RAD17SP
  • RAD24
  • RF-C activator 1 homolog
  • RF-C/activator 1 homolog

Background

The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Eight alternatively spliced transcript variants of this gene, which encode four distinct proteins, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified. [provided by RefSeq]. Transcript Variant: This variant (8) contains a different 5' end region, and lacks an internal 5' UTR region, when compared to variant 1. The encoded protein (isoform 1) is identical to that encoded by variants 1, 5, 6 and 7. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Rad17 Lysate (NBL1-15112) (0)

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Bioinformatics

Gene Symbol RAD17
Entrez