Rad17 [p Ser647] Antibody

Western Blot: Rad17 [p Ser647] Antibody [NB100-56386] - Analysis of Rad17-phos in A) a partial recombinant protein containing phospho Ser647 and B) the same sequence without the phospho serine, and was blocked using the non-phospho control of the immunizing peptide, using this antibody at 1 ug/ml.

Product Discontinued

Rad17 [p Ser647] Antibody Summary

• Immunogen: The antibody was raised against a synthetic peptide corresponding to the amino acids 644-652 (LPLBQNSGS) of mouse Rad17. The B represents a phosphorylated serine residue.
• Modification: p Ser647
• Specificity: RAD17 Phosphospecific [Ser647]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RAD17
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1-1 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS containing 0.2% gelatin
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for Rad17 [p Ser647] Antibody

• CCYC
• cell cycle checkpoint protein (RAD17)
• cell cycle checkpoint protein RAD17
• FLJ41520
• HRAD17
• R24L
• RAD1 (S. pombe) homolog
• RAD1 homolog
• RAD17 homolog (S. pombe)
• Rad17
• Rad17-like protein
• RAD17SP
• RAD24
• RF-C activator 1 homolog
• RF-C/activator 1 homolog

Background

The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe Rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by ATR after the damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA damage induced cell cycle G2 arrest and is thought to be a critical early event during checkpoint signaling in DNA damaged cells. Eight alternatively spliced transcript variants of this gene, which encode four distinct proteins, have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RAD17
• Entrez:
  • 19356(Mouse)
• Uniprot:
  • O75943()