Rad1 Antibody (4126)

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Detection of Rad 1 in HeLa whole cell extracts, using NB 100-346 in Western Blot.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
4126
Clonality
Monoclonal
Host
Mouse
Concentration
1.02 mg/ml

Order Details

Rad1 Antibody (4126) Summary

Immunogen
Amino terminal fragment of human Rad1 purified from E. coli.
Localization
Nuclear
Specificity
This is specific for Rad 1.
Isotype
IgG
Clonality
Monoclonal
Host
Mouse
Gene
RAD1
Purity
Protein G purified
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Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
PBS, 0.2M Tris (pH 7.0), 0.2M Glycine, 1.0mM EGTA and 50% Glycerol
Preservative
0.025% Proclin 300
Concentration
1.02 mg/ml
Purity
Protein G purified

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
Application Notes
WB: Detects an approx. 28 kDa protein representing Rad1 protein. predicted molecular weight: 34 kDa.

Alternate Names for Rad1 Antibody (4126)

  • cell cycle checkpoint protein Hrad1
  • checkpoint control protein HRAD1
  • DNA repair exonuclease rad1 homolog
  • DNA repair exonuclease REC1
  • EC 3.1.11.2
  • exonuclease homolog RAD1
  • HRAD1
  • RAD1 (S. pombe) homolog
  • RAD1 homolog (S. pombe)
  • Rad1-like DNA damage checkpoint protein
  • REC1cell cycle checkpoint protein RAD1

Background

Human Rad1 is a component of a heterotrimeric PCNA-like complex that also contains the Rad9 and Hus1 proteins. This complex is believed to be involved in cellular responses to DNA damage, possibly by associating with Rad17 and several components of the PCNA-loading heteropentamer, replication factor C. Human Rad1 exhibits significant homology to Rad1 from S. pombe, and its expression in yeast rad1 mutants has been shown to partially restore radiation resistance and G2-checkpoint activity. It has also been shown to possess exonuclease activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Rad1 Antibody (NB100-346) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Rad1 Antibody (NB100-346) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on Rad1

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol RAD1
Entrez

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