RAC2 Antibody

Images

 
Western Blot: RAC2 Antibody [NBP1-33449] - Sample (30 ug of whole cell lysate) A: Molt-4 12% SDS PAGE; antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: RAC2 Antibody [NBP1-33449] - Paraffin-embedded mouse fore brain. RAC2 antibody [N1C3] dilution: 1:500.
Immunohistochemistry-Paraffin: RAC2 Antibody [NBP1-33449] - Paraffin-embedded NCI-N87 xenograft, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ch, Dr, RM, Xp, ZeSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.04 mg/ml

Order Details

RAC2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human RAC2. The exact sequence is proprietary.
Localization
Cytoplasm
Predicted Species
Rat (99%), Xenopus (96%), Rhesus Macaque (100%), Zebrafish (96%), Drosophila (92%), Bovine (100%), Chicken (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RAC2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1.04 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RAC2 Antibody

  • Gx
  • HSPC022
  • ras-related C3 botulinum toxin substrate 2 (rho family, small GTP bindingprotein Rac2)
  • rho family, small GTP binding protein Rac2
  • small GTP-bindingprotein Rac2)

Background

RAC2 is a plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, RAC2 binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. There are two isoforms of RAC2; isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RAC2