| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35170-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-27399 | |||
| Azide and BSA Free | NBP2-27399SS | |||
| BSA Free | NB100-56749SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF23151-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70146 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2315-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB23152-SP | |||
| Carrier Free | IC23152U-100UG | |||
| Carrier Free | IC23152T-100UG | |||
| Carrier Free | IC23152S-100UG | |||
| Carrier Free | IC23152R-100UG | |||
| Carrier Free | IC23152N-100UG | |||
| Carrier Free | IC23152G-100UG | |||
| Carrier Free | IC23152V-100UG | |||
![Immunohistochemistry: RAC2 Antibody [NBP3-35170] - Immunohistochemistry analysis of paraffin-embedded Rat brain using RAC2 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35170_rabbit-rac2-pab-912202415143627.jpg "Immunohistochemistry: RAC2 Antibody [NBP3-35170] - Immunohistochemistry analysis of paraffin-embedded Rat brain using RAC2 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunohistochemistry: RAC2 Antibody [NBP3-35170] - Immunohistochemistry analysis of paraffin-embedded Human esophagus using RAC2 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35170_rabbit-rac2-pab-912202414163033.jpg)
![Western Blot: RAC2 Antibody [NBP3-35170] - Western blot analysis of various lysates, using RAC2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-35170_rabbit-rac2-pab-91220241519516.jpg)
![Immunohistochemistry: RAC2 Antibody [NBP3-35170] - Immunohistochemistry analysis of paraffin-embedded Mouse stomach using RAC2 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35170_rabbit-rac2-pab-912202415121394.jpg)
![Immunohistochemistry-Paraffin: RAC2 Antibody [NBP1-33449] - Mouse fore brain. RAC2 antibody [N1C3] dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/RAC2-Antibody-Immunohistochemistry-Paraffin-NBP1-33449-img0004.jpg "Immunohistochemistry-Paraffin: RAC2 Antibody [NBP1-33449] - Mouse fore brain. RAC2 antibody [N1C3] dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Immunohistochemistry-Paraffin: RAC2 Antibody [NBP1-33449] - NCI-N87 xenograft, using RAC2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/RAC2-Antibody-Immunohistochemistry-Paraffin-NBP1-33449-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: RAC2 Antibody [NBP1-33449] - RAC2 antibody [N1C3] detects RAC2 protein at cell membrane and cytoplasm by immunofluorescent analysis.Sample: MOLT-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: RAC2 stained by RAC2 antibody [N1C3] (NBP1-33449) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-33449_rabbit-polyclonal-rac2-antibody-910202420122071.jpg)
![Immunohistochemistry-Paraffin: RAC2 Antibody [NBP1-33449] - RAC2 antibody [N1C3] detects RAC2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse spleen.RAC2 stained by RAC2 antibody [N1C3] (NBP1-33449) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-33449_rabbit-polyclonal-rac2-antibody-91020242011452.jpg)
![Western Blot: RAC2 Antibody [NBP1-33449] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RAC2 antibody [N1C3] (NBP1-33449) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33449_rabbit-polyclonal-rac2-antibody-910202420104415.jpg)
![Western Blot: RAC2 Antibody [NBP2-31086] - (0.1ug/ml) staining of Molt4 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/NBP2-31086_Western-Blot-RAC2-Antibody-NBP2-31086-0-1ug-ml-staining-of-Molt4-lysate-35ug-protein-in-RIPA-buffer-Detected-by-chemiluminescence--29320239254014.jpg "Western Blot: RAC2 Antibody [NBP2-31086] - (0.1ug/ml) staining of Molt4 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: RAC2 Antibody [NBP2-31086] - (2ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.](http://images.novusbio.com/fullsize/NBP2-31086_Immunohistochemistry-Paraffin-RAC2-Antibody-NBP2-31086-2ug-ml-staining-of-paraffin-embedded-Human-Tonsil-Steamed-antigen-retrieval-with-Tris-EDTA-buffer-pH-9-HRP-staining--29320239254019.jpg)
![Western Blot: RAC2 Antibody [NBP1-98473] - Mouse Heart Lysate 1.0ug/ml, Gel Concentration: 12%](http://images.novusbio.com/fullsize/RAC2-Antibody-Western-Blot-NBP1-98473-img0002.jpg "Western Blot: RAC2 Antibody [NBP1-98473] - Mouse Heart Lysate 1.0ug/ml, Gel Concentration: 12%")
![Western Blot: Rac1,2,3 Antibody (2J4S6) [NBP3-16547] - Western blot analysis of extracts of various cell lines, using Rac1,2,3 Rabbit mAb (NBP3-16547) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Rac1-2-3-Antibody-2J4S6-Western-Blot-NBP3-16547-img0001.jpg "Western Blot: Rac1,2,3 Antibody (2J4S6) [NBP3-16547] - Western blot analysis of extracts of various cell lines, using Rac1,2,3 Rabbit mAb (NBP3-16547) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Western Blot: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - Total protein from mouse 3T3 cells treated with and without PDGF (50 ng/mL) for the indicated times was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-AKT1 (NBP2-01725) and 2 ug/mL pS473 AKT1 in 1% BSA in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Note the detection of phosphorylated AKT1 in response to PDGF treatment compared to total AKT1 protein.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-104A282-Western-Blot-NB100-56749-img0014.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - Total protein from mouse 3T3 cells treated with and without PDGF (50 ng/mL) for the indicated times was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-AKT1 (NBP2-01725) and 2 ug/mL pS473 AKT1 in 1% BSA in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Note the detection of phosphorylated AKT1 in response to PDGF treatment compared to total AKT1 protein.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - WB of phospho AKT using phospho AKT antibody at 2 ug/mL against untreated (lane 1) and PDGF treated (lane 2) NIH-3T3 lysate. HRP conjugated secondary antibody and ECL substrate solution were used for this test. Image using the Azide and BSA Free form of this antibody.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-104A282-Western-Blot-NB100-56749-img0015.jpg)
![Immunohistochemistry-Paraffin: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - IHC analysis of an FFPE human breast carcinoma tissue section using 1:250 dilution of phospho Ser473 AKT1 antibody (clone 104A282) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10 mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This antibody generated a diffused cytoplasmic staining of phosphor-AKT (Ser-473) in the cancer cells as well as the stromal cells. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-104A282-Immunohistochemistry-Paraffin-NB100-56749-img0009.jpg)
![Immunohistochemistry-Frozen: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - Rat mammary carcinoma tissue section stained with AKT1 [p Ser473] Antibody (104A282). IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-104A282-Immunohistochemistry-Frozen-NB100-56749-img0016.jpg)
![Immunohistochemistry-Paraffin: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - IHC analysis of an FFPE human breast carcinoma tissue section using 1:250 dilution of pSer473 AKT1 antibody (clone 104A282) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10 mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This antibody generated a diffused cytoplasmic staining of phosphor-AKT (Ser-473) in the cancer cells as well as the stromal cells. Some cancer cells depicted nuclear stianing also. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-104A282-Immunohistochemistry-Paraffin-NB100-56749-img0010.jpg)
![EZH2/HMGA2 regulates the PI3K/AKT signaling. MI rats were subjected to injection of EZH2-OE or EZH2-NC (MI + MSC-EXO + EZH2 NC/EZH2 OE) and EZH2-OE + HMGA2-OE or EZH2-OE + HMGA2-NC. A HMGA2 protein expression in myocardial tissues in response to EZH2-OE + HMGA2-OE examined using western blot (Additional file 5: Fig. S5). B Weight change in rats after EZH2-OE + HMGA2-OE treatment. C Changes in HMI in rats after EZH2-OE + HMGA2-OE treatment. D Measurement of Dd, Sd, and LVEF by echocardiography at 2 weeks after LAD in rats. E Measurement of LVMI by echocardiography in rats. F KEGG pathway analysis of gene enrichment pathways [29]. G Changes in PI3K/AKT pathway in rat myocardium examined using western blot (Additional file 6: Fig. S6). All data are expressed as mean +/- SD (n = 6/group, *p < 0.05 vs. Sham, DMSO, MI + MSC-EXO + EZH2-NC or EZH2-OE + HMGA2-NC group determined by unpaired t test or one-way ANOVA) Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35264108), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56749_mouse-monoclonal-akt1-p-ser473-antibody-104a282-western-blot-13220261820240.jpg)
![Western Blot: AKT2 Antibody (4H7) [NBP2-02231] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT2.](http://images.novusbio.com/fullsize/AKT2-Antibody-4H7-Western-Blot-NBP2-02231-img0003.jpg "Western Blot: AKT2 Antibody (4H7) [NBP2-02231] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT2.")
![Immunocytochemistry/Immunofluorescence: AKT2 Antibody (4H7) [NBP2-02231] Staining of COS7 cells transiently transfected by pCMV6-ENTRY AKT2.](http://images.novusbio.com/fullsize/AKT2-Antibody-4H7-Immunocytochemistry-Immunofluorescence-NBP2-02231-img0007.jpg)
![Immunohistochemistry-Paraffin: AKT2 Antibody (4H7) [NBP2-02231] - Staining of paraffin-embedded Human liver tissue using anti-AKT2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AKT2-Antibody-4H7-Immunohistochemistry-Paraffin-NBP2-02231-img0006.jpg)
![Flow Cytometry: AKT2 Antibody (4H7) [NBP2-02231] - Analysis of Jurkat cells, using anti-AKT2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/AKT2-Antibody-4H7-Flow-Cytometry-NBP2-02231-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: AKT2 Antibody (4H7) [NBP2-02231] - Immunofluorescent staining of HepG2 cells using anti-AKT2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AKT2-Antibody-4H7-Immunocytochemistry-Immunofluorescence-NBP2-02231-img0004.jpg)
![Immunohistochemistry-Paraffin: AKT2 Antibody (4H7) [NBP2-02231] - Staining of paraffin-embedded Human Kidney tissue using anti-AKT2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AKT2-Antibody-4H7-Immunohistochemistry-Paraffin-NBP2-02231-img0005.jpg)
![Flow Cytometry: AKT2 Antibody (4H7) [NBP2-02231] - Analysis of Hela cells, using anti-AKT2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/AKT2-Antibody-4H7-Flow-Cytometry-NBP2-02231-img0001.jpg)
![Western Blot: AKT2 Antibody (X1) [H00000208-M06] - Analysis of AKT2 expression in Jurkat (Cat # L017V1).](http://images.novusbio.com/fullsize/AKT2-Antibody-X1-Western-Blot-H00000208-M06-img0006.jpg "Western Blot: AKT2 Antibody (X1) [H00000208-M06] - Analysis of AKT2 expression in Jurkat (Cat # L017V1).")
![Immunocytochemistry/Immunofluorescence: AKT2 Antibody (X1) [H00000208-M06] - Analysis of monoclonal antibody to AKT2 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/AKT2-Antibody-X1-Immunocytochemistry-Immunofluorescence-H00000208-M06-img0001.jpg)
![Immunohistochemistry-Paraffin: AKT2 Antibody (X1) [H00000208-M06] - Analysis of monoclonal antibody to AKT2 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/AKT2-Antibody-X1-Immunohistochemistry-Paraffin-H00000208-M06-img0002.jpg)
![Western Blot: AKT2 Antibody (X1) [H00000208-M06] - Analysis of AKT2 expression in PC-12 (Cat # L012V1).](http://images.novusbio.com/fullsize/AKT2-Antibody-X1-Western-Blot-H00000208-M06-img0004.jpg)
![Western Blot: AKT2 Antibody (X1) [H00000208-M06] - Analysis of AKT2 expression in Raw 264.7 (Cat # L024V1).](http://images.novusbio.com/fullsize/AKT2-Antibody-X1-Western-Blot-H00000208-M06-img0005.jpg)
![Western Blot: AKT2 Antibody (X1) [H00000208-M06] - Analysis of AKT2 expression in NIH/3T3 (Cat # L018V1).](http://images.novusbio.com/fullsize/AKT2-Antibody-X1-Western-Blot-H00000208-M06-img0007.jpg)
![ELISA: AKT2 Antibody (X1) [H00000208-M06] - Detection limit for recombinant GST tagged AKT2 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/AKT2-Antibody-X1-ELISA-H00000208-M06-img0003.jpg)
![Western Blot: AKT2 Antibody (1D9) [H00000208-M03] - Analysis of AKT2 expression in Jurkat (Cat # L017V1).](http://images.novusbio.com/fullsize/AKT2-Antibody-1D9-Western-Blot-H00000208-M03-img0011.jpg "Western Blot: AKT2 Antibody (1D9) [H00000208-M03] - Analysis of AKT2 expression in Jurkat (Cat # L017V1).")
![Immunocytochemistry/Immunofluorescence: AKT2 Antibody (1D9) [H00000208-M03] - Analysis of monoclonal antibody to AKT2 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/AKT2-Antibody-1D9-Immunocytochemistry-Immunofluorescence-H00000208-M03-img0008.jpg)
![Western Blot: AKT2 Antibody (1D9) [H00000208-M03] - Analysis of AKT2 expression in PC-12 (Cat # L012V1).](http://images.novusbio.com/fullsize/AKT2-Antibody-1D9-Western-Blot-H00000208-M03-img0009.jpg)
![Western Blot: AKT2 Antibody (1D9) [H00000208-M03] - Analysis of AKT2 expression in Raw 264.7 (Cat # L024V1).](http://images.novusbio.com/fullsize/AKT2-Antibody-1D9-Western-Blot-H00000208-M03-img0010.jpg)
![Western Blot: AKT2 Antibody (1D9) [H00000208-M03] - Analysis of AKT2 expression in NIH/3T3 (Cat # L018V1).](http://images.novusbio.com/fullsize/AKT2-Antibody-1D9-Western-Blot-H00000208-M03-img0012.jpg)
![ELISA: AKT2 Antibody (1D9) [H00000208-M03] - Detection limit for recombinant GST tagged AKT2 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/AKT2-Antibody-1D9-ELISA-H00000208-M03-img0006.jpg)