R-Spondin 4 Antibody (435203)

• Reactivity: Mu
• Applications: WB
• Clone: 435203
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

R-Spondin 4 Antibody (435203) Summary

• Immunogen: E. coli-derived recombinant mouse R‑Spondin 4
  Tyr21-Pro197
  Accession # Q8BJ73
• Specificity: Detects mouse R-Spondin 4 in direct ELISAs and Western blots. In Western blots, 50-100% cross-reactivity with recombinant human (rh) R-Spondin 4 is observed and no cross-reactivity with recombinant mouse (rm) R-Spondin 1, rmR-Spondin 3, or rhR-Spondin 2 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: RSPO4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for R-Spondin 4 Antibody (435203)

• C20orf182
• chromosome 20 open reading frame 182
• Cristin 4
• CRISTIN4
• dJ824F16.3
• FLJ16018
• hRspo4
• Roof plate-specific spondin-4
• RSPO4
• RSpondin 4
• R-Spondin 4
• R-spondin family, member 4
• R-spondin-4

Background

R-Spondin 4 (RSPO4, roof plate-specific spondin 4), also called cysteine-rich and single thrombospondin domain containing-4 (Cristin 4), is an ~33 kDa secreted heparin-binding protein that shares ~35% amino acid (aa) identity with three other R-Spondin family members (1-3). All are positive modulators of Wnt/ beta -catenin signaling, but R-Spondin 4 may be somewhat weaker than other R-Spondins (2). R-Spondins regulate Wnt/ beta -catenin by competing with the Wnt antagonist DKK-1 for binding to the Wnt co-receptors LRP-6 and Kremen, reducing their DKK-1-mediated internalization (1, 4). Like other R-Spondins, mouse R-Spondin 4 (234 aa) contains a signal sequence (aa 1-19), two adjacent cysteine-rich furin-like domains (aa 85-128) with one potential tyrosine phosphorylation site (aa 114), followed by a thrombospondin (TSP-1) motif (aa 137-197) and a region rich in basic residues (aa 199-234). The furin-like domains are sufficient for beta -catenin stabilization (2). Mature mouse R-Spondin 4 shares 81%, 97%, 79%, 77% and 76% aa identity with human, rat, bovine, equine and canine R-Spondin 4, respectively. There is one potential isoform where Arg substitutes for the C-terminal 82 amino acids (5). Each R-Spondin has a distinct expression pattern (6). In the mouse, R-Spondin 4 mRNA is found during development of limb bud mesenchyme, nail beds, heart and teeth (6-8). In humans, mutations of R-Spondin 4 have been found to cause anonychia, a condition in which fingernails and toenails are absent (8-10).

1. Nam, J.-S. et al. (2006) J. Biol. Chem. 281:13247.
2. Kim, K.-A. et al. (2008) Mol. Biol. Cell 19:2588.
3. Hendrickx, M. and L. Leyns (2008) Develop. Growth Differ. 50:229.
4. Binnerts, M.E. et al. (2007) Proc. Natl. Acad. Sci. USA 104:14700.
5. Entrez Accession # Q8BJ73, Isoform 2.
6. Nam, J.-S. et al. (2007) Gene Expr. Patterns 7:306.
7. Pemberton, T.J. et al. (2007) Dev. Dyn. 236:2245.
8. Ishii, Y. et al. (2008) J. Invest. Dermatol. 128:867.
9. Blaydon, D.C. et al. (2006) Nat. Genet. 38:1245.
10. Bergmann, C. et al. (2006) Am. J. Hum. Genet. 79:1105.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RSPO4
• Uniprot:
  • Q8BJ73(Mouse)