Plakophilin 1 Antibody

Images

 
Western Blot: Plakophilin 1 Antibody [NBP1-90042] - Analysis using Anti-PKP1 antibody NBP1-90042 (A) shows similar pattern to independent antibody NBP1-90041 (B).
Immunocytochemistry/ Immunofluorescence: Plakophilin 1 Antibody [NBP1-90042] - Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm & plasma membrane.
Orthogonal Strategies: Immunohistochemistry-Paraffin: Plakophilin 1 Antibody [NBP1-90042] - Staining in human skin and skeletal muscle tissues using anti-PKP1 antibody. Corresponding PKP1 RNA-seq data are ...read more
Western Blot: Plakophilin 1 Antibody [NBP1-90042] - Analysis in human cell line PC-3.
Western Blot: Plakophilin 1 Antibody [NBP1-90042] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Independent Antibodies: Western Blot: Plakophilin 1 Antibody [NBP1-90042] - Analysis in human cell lines PC-3 and A-549 using anti-PKP1 antibody. Corresponding PKP1 RNA-seq data are presented for the same cell ...read more
Immunohistochemistry-Paraffin: Plakophilin 1 Antibody [NBP1-90042] - Staining of human oral mucosa shows strong cytoplasmic, membranous and nuclear positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: Plakophilin 1 Antibody [NBP1-90042] - Staining of human skeletal muscle shows low expression as expected.
Immunohistochemistry-Paraffin: Plakophilin 1 Antibody [NBP1-90042] - Staining of human skin shows high expression.

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Plakophilin 1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: YTVRNLMASQPQLAKQYFSSSMLNNIINLCRSSASPKAAEAARLLLSDMWSSKELQGVLRQQGFDRNMLGTLAGANSLRNFTSRF
Specificity
Specificity of human, rat Plakophilin 1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PKP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Plakophilin 1 Protein (NBP1-90042PEP)
Publications
Read Publication using NBP1-90042.

Reactivity Notes

Reactivity reported in scientific literature (PMID: 22170739). Mouse (84%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Plakophilin 1 Antibody

  • B6PBand 6 protein
  • MGC138829
  • plakophilin 1 (ectodermal dysplasia/skin fragility syndrome)
  • plakophilin-1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Plakophilin 1 Antibody (NBP1-90042)(1)

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Diseases for Plakophilin 1 Antibody (NBP1-90042)

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Bioinformatics

Gene Symbol PKP1