ZNF447 Antibody (OTI2H7)

Images

 
Western Blot: ZNF447 Antibody (OTI2H7) [NBP2-45442] - Analysis of extracts (35ug) from 9 different cell lines by usin g ZSCAN18 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; ...read more
Immunohistochemistry: ZNF447 Antibody (OTI2H7) [NBP2-45442] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: ZNF447 Antibody (OTI2H7) [NBP2-45442] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZNF447.
Immunohistochemistry: ZNF447 Antibody (OTI2H7) [NBP2-45442] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: ZNF447 Antibody (OTI2H7) [NBP2-45442] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: ZNF447 Antibody (OTI2H7) [NBP2-45442] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: ZNF447 Antibody (OTI2H7) [NBP2-45442] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: ZNF447 Antibody (OTI2H7) [NBP2-45442] - Analysis of Human lung tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: ZNF447 Antibody (OTI2H7) [NBP2-45442] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: ZNF447 Antibody (OTI2H7) [NBP2-45442] - Analysis of Carcinoma of Human kidney tissue.
Immunohistochemistry: ZNF447 Antibody (OTI2H7) [NBP2-45442] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunofluorescence: ZNF447 Antibody (OTI2H7) [NBP2-45442] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY ZNF447.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, PmSpecies Glossary
Applications WB, ICC/IF, IHC, ICC/IF
Clone
OTI2H7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

ZNF447 Antibody (OTI2H7) Summary

Immunogen
Full lenth human recombinant protein of human ZSCAN18(NP_076415) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ZSCAN18
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Western Blot 1:2000-4000
Theoretical MW
54.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ZNF447 Antibody (OTI2H7)

  • DKFZp586B1122
  • FLJ12895
  • FLJ38919
  • FLJ44152
  • MGC4074
  • MGC8682
  • zinc finger and SCAN domain containing 18
  • zinc finger and SCAN domain-containing protein 18
  • Zinc finger protein 447MGC2427
  • ZNF447

Background

ZNF447 may be involved in transcriptional regulation

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol ZSCAN18