Claudin-6 Antibody (342927) [Unconjugated]

F0101B). Quadrant markers were set based on Mouse IgG2B Isotype Control Antibody (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins." > F0101B). Quadrant markers were set based on Mouse IgG2B Isotype Control Antibody (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins." title="HEK293 human cell line transfected with (A) Human Claudin-6 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human Claudin-6 Monoclonal Antibody (Catalog # MAB3656) followed by APC-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on Mouse IgG2B Isotype Control Antibody (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins." />

HEK293 human cell line transfected with (A) Human Claudin-6 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human Claudin-6 Monoclonal Antibody (Catalog # MAB3656) followed by APC-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # <a class=

SC019), using Mouse Anti-Human Claudin-6 Monoclonal Antibody (Catalog # MAB3656) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). SOX17 was also detected using Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (green; Catalog # AF1924). Specific staining of Claudin-6 was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips." > SC019), using Mouse Anti-Human Claudin-6 Monoclonal Antibody (Catalog # MAB3656) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). SOX17 was also detected using Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (green; Catalog # AF1924). Specific staining of Claudin-6 was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips." title="Claudin-6 was detected in immersion fixed iPS2 human induced pluripotent stem cells, differentiated into definitive endoderm with StemXVivo Endoderm Kit (Catalog # SC019), using Mouse Anti-Human Claudin-6 Monoclonal Antibody (Catalog # MAB3656) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). SOX17 was also detected using Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (green; Catalog # AF1924). Specific staining of Claudin-6 was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips." />

Claudin-6 was detected in immersion fixed iPS2 human induced pluripotent stem cells, differentiated into definitive endoderm with StemXVivo Endoderm Kit (Catalog # <a class=

• Applications: Flow, CyTOF-ready, ICC/IF
• Clone: 342927
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Claudin-6 Antibody (342927) [Unconjugated] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human Claudin-6
  Met1-Val220
  Accession # P56747
• Specificity: Stains human Claudin-6 transfected cells but not irrelevant transfectants.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CLDN6
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Claudin-6 Antibody (342927) [Unconjugated]

• claudin 6
• Claudin6
• Claudin-6
• CLDN6
• Skullin 2
• skullin

Background

Claudin-6 is a multipass transmembrane protein in the Claudin family. Claudin-6 is expressed by epithelial cells where it participates in tissue development and the maintenance of tight junction integrity. Human Claudin-6 shares 88% and 86% amino acid sequence identity with mouse and rat Claudin-6, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Claudin-6 Antibody (MAB3656)(4)

Publications using MAB3656

• Mei-Mei Li, Fan-En Kong, Guang-Meng Li, Yi-Ti He, Xiao-Feng Zhang, Cheng-Yang Zhang et al. Identification and functional characterization of potential oncofetal targets in human hepatocellular carcinoma STAR Protocols 2022-12-16 [PMID: 36595904]
• Brad Screnci, Lewis J. Stafford, Trevor Barnes, Kristen Shema, Samantha Gilman, Rebecca Wright et al. Antibody specificity against highly conserved membrane protein Claudin 6 driven by single atomic contact point iScience 2022-12-22 [PMID: 36505931]
• Y Shirasago, H Fukazawa, S Nagase, Y Shimizu, T Mizukami, T Wakita, T Suzuki, H Tani, M Kondoh, T Kuroda, S Yasuda, Y Sato, K Hanada, M Fukasawa A single mutation in the E2 glycoprotein of hepatitis C virus broadens the claudin specificity for its infection Scientific Reports, 2022-11-24;12(1):20243. 2022-11-24 [PMID: 36424447] (Bioassay, Human)
• J Haddad, Y Rouillé, X Hanoulle, V Descamps, M Hamze, F Dabboussi, TF Baumert, G Duverlie, M Lavie, J Dubuisson Identification of novel functions for hepatitis C virus envelope glycoprotein E1 in virus entry and assembly J. Virol, 2017-03-29;0(0):. 2017-03-29 [PMID: 28179528] (Flow Cytometry, Functional Assay, Human)

Bioinformatics

• Gene Symbol: CLDN6
• Uniprot:
  • P56747()