R-Spondin 4 Antibody (435203) [Alexa Fluor® 594]

• Reactivity: Mu
• Applications: WB
• Clone: 435203
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 594

R-Spondin 4 Antibody (435203) [Alexa Fluor® 594] Summary

• Immunogen: E. coli-derived recombinant mouse R‑Spondin 4
  Tyr21-Pro197
  Accession # Q8BJ73
• Specificity: Detects mouse R‑Spondin 4 in direct ELISAs and Western blots. In Western blots, 50‑100% cross‑reactivity with recombinant human (rh) R‑Spondin 4 is observed and no cross-reactivity with recombinant mouse (rm) R‑Spondin 1, rmR
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for R-Spondin 4 Antibody (435203) [Alexa Fluor® 594]

• C20orf182
• chromosome 20 open reading frame 182
• Cristin 4
• CRISTIN4
• dJ824F16.3
• FLJ16018
• hRspo4
• Roof plate-specific spondin-4
• RSPO4
• RSpondin 4
• R-Spondin 4
• R-spondin family, member 4
• R-spondin-4

Background

R-Spondin 4 (RSPO4, roof plate-specific spondin 4), also called cysteine-rich and single thrombospondin domain containing-4 (Cristin 4), is an ~33 kDa secreted heparin-binding protein that shares ~35% amino acid (aa) identity with three other R-Spondin family members (1-3). All are positive modulators of Wnt/ beta -catenin signaling, but R-Spondin 4 may be somewhat weaker than other R-Spondins (2). R‑Spondins regulate Wnt/ beta -catenin by competing with the Wnt antagonist DKK-1 for binding to the Wnt co-receptors LRP-6 and Kremen, reducing their DKK‑1‑mediated internalization (1, 4). Like other R‑Spondins, mouse R-Spondin 4 (234 aa) contains a signal sequence (aa 1-19), two adjacent cysteine-rich furin-like domains (aa 85-128) with one potential tyrosine phosphorylation site (aa 114), followed by a thrombospondin (TSP-1) motif (aa 137‑197) and a region rich in basic residues (aa 199‑234). The furin-like domains are sufficient for beta -catenin stabilization (2). Mature mouse R‑Spondin 4 shares 81%, 97%, 79%, 77% and 76% aa identity with human, rat, bovine, equine and canine R-Spondin 4, respectively. There is one potential isoform where Arg substitutes for the C‑terminal 82 amino acids (5). Each R‑Spondin has a distinct expression pattern (6). In the mouse, R‑Spondin 4 mRNA is found during development of limb bud mesenchyme, nail beds, heart and teeth (6‑8). In humans, mutations of R‑Spondin 4 have been found to cause anonychia, a condition in which fingernails and toenails are absent (8‑10).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.