QPRT Antibody - BSA Free

Images

 
Immunohistochemistry-Paraffin: QPRT Antibody [NBP3-17089] - Analysis in human liver and skeletal muscle tissues using Anti-QPRT antibody. Corresponding QPRT RNA-seq data are presented for the same tissues.
Western Blot: QPRT Antibody [NBP3-17089] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: RT4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: Tonsil
Immunocytochemistry/ Immunofluorescence: QPRT Antibody [NBP3-17089] - Staining of human cell line Hep G2 shows localization to vesicles.
Immunohistochemistry-Paraffin: QPRT Antibody [NBP3-17089] - Staining of human skeletal muscle shows low expression as expected.
Immunohistochemistry-Paraffin: QPRT Antibody [NBP3-17089] - Staining of human liver shows high expression.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free

Order Details

QPRT Antibody - BSA Free Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LLDNFKPEELHPTATVLKAQFPSVAVEASGGITLDNLPQFCGPHIDVISMGMLTQAAPALDFSLKLFAKEVAPVPKI
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
QPRT
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Reactivity Notes

Mouse (81%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for QPRT Antibody - BSA Free

  • EC 2.4.2.19
  • nicotinate-nucleotide pyrophosphorylase (carboxylating)
  • QAPRTase
  • QPRTasenicotinate-nucleotide pyrophosphorylase [carboxylating]
  • Quinolinate phosphoribosyltransferase [decarboxylating]
  • quinolinate phosphoribosyltransferase

Background

QPRT encodes a key enzyme in catabolism of quinolinate, an intermediate in the tryptophan-nicotinamide adenine dinucleotide pathway. Quinolinate acts as a most potent endogenous exitotoxin to neurons. Elevation of quinolinate levels in the brain has been linked to the pathogenesis of neurodegenerative disorders such as epilepsy, Alzheimer's disease, and Huntington's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol QPRT