Pygopus-1 Antibody

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Pygopus-1 Antibody Summary

• Immunogen: E. coli-derived recombinant mouse Pygopus-1
  Ser2-Ala417
  Accession # Q9D0P5
• Specificity: Detects mouse Pygopus-1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PYGO1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Pygopus-1 Antibody

• DKFZp547G0910
• Pygo1
• pygopus 1
• pygopus homolog 1 (Drosophila)
• pygopus homolog 1
• Pygopus1
• Pygopus-1
• pygopus-like protein 1

Background

Pygopus (the name of a legless Australian lizard) is a 45 kDa transcription co-activator that functions in the Wnt signaling pathway. Mouse Pygopus-1 is a nuclear protein that is synthesized as a 417 amino acid residue protein that has the conserved N-terminal homology domain (NHD) and the C-terminal PHD (Plant homeodomain) finger Zn²⁺-binding domain. A nuclear localization signal is present within the NHD. The PHD finger of Pygopus binds to Legless/Bcl9, which in turn binds beta -Catenin, which recruits TCF/LEF DNA binding proteins. Formation of the quaternary complex is required for Wnt signaling. Mouse and human Pygopus-1 share 87% amino acid sequence homology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PYGO1
• Uniprot:
  • Q9D0P5()