Pygopus-1 Antibody [Alexa Fluor® 350]

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 350

Pygopus-1 Antibody [Alexa Fluor® 350] Summary

• Immunogen: E. coli-derived recombinant mouse Pygopus-1
  Ser2-Ala417
  Accession # Q9D0P5
• Specificity: Detects mouse Pygopus-1 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Pygopus-1 Antibody [Alexa Fluor® 350]

• DKFZp547G0910
• Pygo1
• pygopus 1
• pygopus homolog 1 (Drosophila)
• pygopus homolog 1
• Pygopus1
• Pygopus-1
• pygopus-like protein 1

Background

Pygopus (the name of a legless Australian lizard) is a 45 kDa transcription co-activator that functions in the Wnt signaling pathway. Mouse Pygopus-1 is a nuclear protein that is synthesized as a 417 amino acid residue protein that has the conserved N-terminal homology domain (NHD) and the C-terminal PHD (Plant homeodomain) finger Zn²⁺-binding domain. A nuclear localization signal is present within the NHD. The PHD finger of Pygopus binds to Legless/Bcl9, which in turn binds beta -Catenin, which recruits TCF/LEF DNA binding proteins. Formation of the quaternary complex is required for Wnt signaling. Mouse and human Pygopus-1 share 87% amino acid sequence homology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.