PYGL Antibody - BSA Free

Western Blot: PYGL Antibody [NBP2-32246] - Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-PYGL antibody NBP2-32246 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoprecipitation: PYGL Antibody [NBP2-32246] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using RIPA buffer from 293T cells. Antibodies: Affinity purified rabbit anti-PYGL antibody NBP2-32246 used for IP at 6 ug per reaction. PYGL was also immunoprecipitated by rabbit anti-PYGL antibody BL15846. For blotting immunoprecipitated PYGL, NBP2-32246 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu, Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

PYGL Antibody - BSA Free Summary

• Immunogen: The immunogen this antibody was made to, maps to a region between residue 797 to 847 of human Phosphorylase, Glycogen, Liver using the numbering given in entry NP_002854.3 (GeneID 5836).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PYGL
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2 - 10 ug/mg lysate
  • Western Blot 1:2000 - 1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PYGL Antibody - BSA Free

• EC 2.4.1.1
• glycogen phosphorylase, liver form
• GSD6
• phosphorylase, glycogen, liver
• phosphorylase, glycogen; liver
• PYGL

Background

Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. Activity of phosphorylase is controlled both by allosteric means (through the noncovalent binding of metabolites) and by covalent modification. Thus AMP allosterically activates, whereas ATP, ADP, and glucose 6 phosphate allosterically inhibit, phosphorylase B.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PYGL Antibody (NBP2-32246)(1)

Publication using NBP2-32246

• Abulizi A, Camporez J P et al. Adipose glucocorticoid action influences whole-body metabolism via modulation of hepatic insulin action. FASEB J 2019-03-28 [PMID: 30922125] (WB, Mouse)

Bioinformatics

• Gene Symbol: PYGL
• Uniprot:
  • NP_002854.3(Human)