Glycogen phosphorylase, muscle form Antibody - BSA Free

Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] - Region-based patterns of glycogen phosphorylase, muscle form (GPmm) Protein expression inicvLz-pretreated male and female rats. GPmm protein was measured by Western blot in the rostral (a; F(7,40)=35.87; pSample: Paraffin-embedded breast cancer. PYGM antibody [C1C3] (NBP2-16689) dilution: 1:500. br>Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min

• Reactivity: Hu, Mu, Ze, Bv, Rb, RM, Sh
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Glycogen phosphorylase, muscle form Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Glycogen phosphorylase, muscle form Antibody - BSA Free (NBP2-16689) is a polyclonal antibody validated for use in IHC and WB. Anti-Glycogen phosphorylase, muscle form Antibody: Cited in 13 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Glycogen phosphorylase, muscle form. The exact sequence is proprietary.
• Predicted Species: Bovine (96%), Rabbit (95%), Rhesus Macaque (98%), Sheep (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PYGM
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 97 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (84%). Rat reactivity reported in scientific literature (PMID: 30660767).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Glycogen phosphorylase, muscle form Antibody - BSA Free

• EC 2.4.1.1
• Glycogen phosphorylase, muscle form
• Myophosphorylase
• phosphorylase, glycogen, muscle
• phosphorylase, glycogen; muscle
• PYGM

Background

Glycogen phosphorylase (EC 2.4.1.1) catalyzes and regulates the breakdown of glycogen to glucose-1-phosphate.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Glycogen phosphorylase, muscle form Antibody (NBP2-16689)(13)

Publications using NBP2-16689

• Briski KP, Napit PR, Alhamyani A et al. Sex-Dimorphic Octadecaneuropeptide (ODN) Regulation of Ventromedial Hypothalamic Nucleus Glucoregulatory Neuron Function and Counterregulatory Hormone Secretion ASN neuro 2023-05-17 [PMID: 37194319] (WB, Rat)
• Briski KP, Mahmood ASMH, Uddin MM et al. Effects of Ventromedial Hypothalamic Nucleus (VMN) Aromatase Gene Knockdown on VMN Glycogen Metabolism and Glucoregulatory Neurotransmission Biology 2023-02-03 [PMID: 36829519] (Western Blot, Rat)
• Pasula MB, Napit PR, Alhamyani A et al. Sex Dimorphic Glucose Transporter-2 Regulation of Hypothalamic Astrocyte Glucose and Energy Sensor Expression and Glycogen Metabolism Neurochemical research 2022-09-29 [PMID: 36173588]
• Alhamyani A, Mahmood ASMH, Alshamrani A et al. Central Type II Glucocorticoid Receptor Regulation of Ventromedial Hypothalamic Nucleus Glycogen Metabolic Enzyme and Glucoregulatory Neurotransmitter Marker Protein Expression in the Male Rat J Endocrinol Diabetes 2021-01-13 [PMID: 34258390] (Western Blot, Rat)
• Bheemanapally K, Alhamyani A, Alshamrani AA et al. Hypoglycemic and post?hypoglycemic patterns of glycogen phosphorylase isoform expression in the ventrolateral ventromedial hypothalamic nucleus: impact of sex and estradiol Acta Neurobiologiae Experimentalis 2021-01-01 [PMID: 34170267] (Western Blot, Rat)
• Briski K, Napit P, Haider Ali M et al. Hindbrain Catecholamine Regulation of Ventromedial Hypothalamic Nucleus Glycogen Metabolism during Acute Versus Recurring Insulin-Induced Hypoglycemia in Male versus Female Rat Endocr Metab Sci 2021-05-17 [PMID: 33997825]
• Uddin MM, Ibrahim MMH, Briski KP Sex-dimorphic neuroestradiol regulation of ventromedial hypothalamic nucleus glucoregulatory transmitter and glycogen metabolism enzyme protein expression in the rat BMC Neurosci 2020-11-25 [PMID: 33238883] (Western Blot, Rat)
• Ibrahim MMH, Bheemanapally K, Sylvester PW, Briski KP. Norepinephrine Regulation of Adrenergic Receptor Expression, 5' AMP-Activated Protein Kinase Activity, and Glycogen Metabolism and Mass in Male Versus Female Hypothalamic Primary Astrocyte Cultures ASN Neuro 2020-11-12 [PMID: 33176438] (Western Blot, Rat)
• Mahmood A S M H, Napit P R et al. Estrogen Receptor Involvement in Noradrenergic Regulation of Ventromedial Hypothalamic Nucleus Glucoregulatory Neurotransmitter and Stimulus-Specific Glycogen Phosphorylase Enzyme Isoform Expression. ASN Neuro 2020-03-04 [PMID: 32233668] (WB, Rat)
• Briski, K P & Mandal, S K. Hindbrain metabolic deficiency regulates ventromedial hypothalamic nucleus glycogen metabolism and glucose regulatory signaling. Acta Neurobiol Exp (Wars) 2020-03-28 [PMID: 32214275] (IF/IHC, Green monkey)
• Bheemanapally K, Ibrahim MMH, Briski KP Combinatory high-resolution microdissection/ultra performance liquid chromatographic-mass spectrometry approach for small tissue volume analysis of rat brain glycogen J Pharm Biomed Anal 2019-09-30 [PMID: 31606560] (WB, Rat)
• Briski K, Mandal S Hindbrain lactoprivic regulation of hypothalamic neuron transactivation and gluco-regulatory neurotransmitter expression: Impact of antecedent insulin-induced hypoglycemia Neuropeptides 2019-08-01 [PMID: 31488323] (WB, Rat)
• Hasan Mahmood ASM, Mandal SK, Bheemanapally K et al. Norepinephrine control of ventromedial hypothalamic nucleus glucoregulatory neurotransmitter expression in the female rat: Role of monocarboxylate transporter function. Mol. Cell. Neurosci. 2019-01-17 [PMID: 30660767] (WB, Rat)

Bioinformatics

• Gene Symbol: PYGM
• Uniprot:
  • P11217(Human)