CRABP2 Antibody

Western Blot: CRABP2 Antibody [NBP2-15998] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with CRABP2 antibody [N1C3] diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: CRABP2 Antibody [NBP2-15998] - HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CRABP2 protein stained by CRABP2 antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: CRABP2 Antibody [NBP2-15998] - Paraffin-Embedded adult mouse retina. Green: CRABP2 protein stained by CRABP2 antibody [N1C3] diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [11710] diluted at 1:500. Blue: Fluoroshield with DAPI.

Western Blot: CRABP2 Antibody [NBP2-15998] - Sample (30 ug of whole cell lysate) A: Hep G2 15% SDS PAGE NBP2-15998 diluted at 1:1000.

Immunohistochemistry-Paraffin: CRABP2 Antibody [NBP2-15998] - Immunohistochemical analysis of paraffin-embedded MDAMB468 xenograft, using antibody at 1:500 dilution.

• Reactivity: Hu, Mu, Rt, Po, Bv, RM
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

CRABP2 Antibody Summary

• Description: Novus Biologicals Rabbit CRABP2 Antibody (NBP2-15998) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human CRABP2. The exact sequence is proprietary.
• Predicted Species: Porcine (95%), Bovine (97%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CRABP2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 16 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (89%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for CRABP2 Antibody

• cellular retinoic acid binding protein 2
• Cellular retinoic acid-binding protein II
• CRABP2
• CRABP-II
• CRABP-IIcellular retinoic acid-binding protein 2
• RBP6

Background

A number of specific carrier proteins for members of the vitamin A family have been discovered. Cellular retinoic acid binding proteins (CRABP) are low molecular weight proteins whose precise function remains unknown. The inducibility of the CRABP2 gene suggests that this isoform is important in retinoic acid-mediated regulation of human skin growth and differentiation. It has been postulated that the CRABP2 gene is transcriptionally regulated by a newly synthesized regulatory protein. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: CRABP2
• Uniprot:
  • P29373()