PXMP4 Antibody

Western Blot: PXMP4 Antibody [NBP3-05927] - Lane A: COLO205 Whole Cell LysateLane B: NCI-H460 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:24 kDaObserved band size:24 kDa

Immunocytochemistry/Immunofluorescence: PXMP4 Antibody [NBP3-05927] - Staining of PXMP4 in CACO2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human PXMP4 polyclonal antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm

Immunohistochemistry-Paraffin: PXMP4 Antibody [NBP3-05927] - Staining of human PXMP4 in human kidney with rabbit polyclonal antibody at 1:100 dilution.

Immunohistochemistry-Paraffin: PXMP4 Antibody [NBP3-05927] - Human PXMP4 in human liver with rabbit polyclonal antibody at 1:100 dilution.

Immunoprecipitation: PXMP4 Antibody [NBP3-05927] - Lane A:0.5 mg COLO205 Whole Cell Lysate4 uL anti-PXMP4 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PXMP4 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 24 kDaObserved band size :24 kDa

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

PXMP4 Antibody Summary

• Description: This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human PXMP4 fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PXMP4
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1-5uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for PXMP4 Antibody

• PMP24
• PXMP4 peroxisomal membrane protein 4, 24kDa

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PXMP4