Home » PXMP2 » PXMP2 Lysates » PXMP2 Overexpression Lysate

PXMP2 Overexpression Lysate

 Submit a Review

Images

 
(
Enlarge)
Western Blot: PXMP2 Overexpression Lysate (Adult Normal) [NBL1-15012] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PXMP2.
Western Blot: PXMP2 Overexpression Lysate (Adult Normal) [NBL1-15012] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PXMP2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

100% Guarantee on every product

PXMP2 Overexpression Lysate Summary

Description

PXMP2 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC209935

Accession#: NM_018663

Protein Tag: C-MYC/DDK
Gene
PXMP2

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
22.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for PXMP2 Overexpression Lysate

  • FLJ54922
  • peroxisomal membrane protein 2 (22kD)
  • peroxisomal membrane protein 2
  • peroxisomal membrane protein 2, 22kDa
  • PMP2222 kDa peroxisomal membrane protein

Background

Seems to be involved in pore-forming activity and may contribute to the unspecific permeability of the peroxisomal membrane

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Myelin Protein
Zero Antibody
NB100-1607
Immunohistochemistry: Myelin Protein Zero Antibody [NB100-1607] - In the image (a lower power tissue section through an adult sciatic nerve), Myelin Protein Zero (brown staining) can be seen in all of the myelinating Schwann cells.
Immunocytochemistry/Immunofluorescence: Myelin Protein Zero Antibody [NB100-1607] - Immunohistochemical staining of P-zero on a 4.0% paraformaldehyde-fixed, paraffin-embedded tissue section through the sciatic nerve of an adult mouse. (Green) P0 concentration was 1:1000 dilution, followed by fluorescein-labeled goat anti-chicken IgY (1:500 dilution). Red is actin. Blue is DAPI nuclear counterstain.
(9 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IP, WB
Connexin 32/GJB1
Antibody (M12.13)
NBP2-53381
Western Blot: Connexin 32/GJB1 Antibody (M12.13) [NBP2-53381] - Western Blot Analysis of human stomach lysate using Connexin 32/GJB1 Antibody (M12.13).
(1 Publication)
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, WB
GDI1 Antibody
NBP1-31528
Western Blot: GDI1 Antibody [NBP1-31528] - Sample (30 ug of whole cell lysate) A:NIH-3T3 10% SDS PAGE; antibody diluted at 1:1000.
Western Blot: GDI1 Antibody [NBP1-31528] - Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE; antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: GDI1 Antibody [NBP1-31528] - Sample: Paraffin-embedded U87 xenograft. GDI1 antibody dilution: 1:200.
Species: Hu, Mu
Applications: IHC, IHC-P, WB
RPLP0 Antibody
NBP1-82859
Immunohistochemistry-Paraffin: RPLP0 Antibody [NBP1-82859] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: RPLP0 Antibody [NBP1-82859] - Staining of human skin shows weak to moderate cytoplasmic positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: RPLP0 Antibody [NBP1-82859] - Staining of human fallopian tube shows weak cytoplasmic positivity in glandular cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
EGR2 Antibody -
BSA Free
NB110-59723
Immunocytochemistry/Immunofluorescence: EGR2 Antibody [NB110-59723] - MCF7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-EGR2 Antibody NB110-59723 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Immunohistochemistry-Paraffin: EGR2 Antibody [NB110-59723] - Analysis of a FFPE tissue section of mouse brain using 1:200 dilution of EGR2 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.
Western Blot: EGR2 Antibody [NB110-59723] - Detection of EGR2 in human fetal lung tissue using NB110-59723.
(1 Review)
(1 Publication)
Species: Hu, Mu, Po
Applications: ICC/IF, IHC, IHC-P, WB
KIF1B Antibody
NB100-57493
Western Blot: KIF1B Antibody [NB100-57493] - Detection of Human and Mouse KIF1B on HeLa whole cell lysate using NB100-57493. KIF1B was also immunoprecipitated by rabbit anti-KIF1B antibody NB100-57494.
(3 Publications)
Species: Hu, Mu
Applications: IP, WB
MBP Antibody (12)
NB600-717
Immunohistochemistry-Frozen: MBP Antibody (12) [NB600-717] - Staining of MBP in mouse spinal cord ventral white matter. Image from verified customer review.
Immunohistochemistry-Frozen: MBP Antibody (12) [NB600-717] - Staining of MBP in mouse brain corpus callosum. Image from verified customer review.
Immunocytochemistry/Immunofluorescence: MBP Antibody (12) [NB600-717] - Histological and mRNA analysis of the inflammatory cytokines in the vehicle- or etifoxine-treated mice at onset of clinical symptoms. At day 10 p.i., drug treated animals showed significant differences in MBP staining. Animals treated with etifoxine showed an increase in retention of percentage of MBP coverage (*p = 0.001). Image collected and cropped by CiteAb from the following publication (http://embomolmed.embopress.org/cgi/doi/10.1002/emmm.201202124), licensed under a CC-BY licence.
(3 Reviews)
(29 Publications)
Species: Bv, Ca, Ch, Fe, Gp, Hu, Ma, Mu, Po, Rb, Rt, Sh
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, RI, WB
MAG/Siglec-4a
538-MG
MAG/Siglec-4a
(19 Publications)
Species: Rt
Applications: BA
MAD2L1-binding
protein Antibody (4G1
H00009587-M03
Immunocytochemistry/Immunofluorescence: MAD2L1-binding protein Antibody (4G11) [H00009587-M03] - Analysis of monoclonal antibody to MAD2L1BP on HeLa cell. Antibody concentration 10 ug/ml
Western Blot: MAD2L1-binding protein Antibody (4G11) [H00009587-M03] - Analysis of MAD2L1BP expression in Hela S3 NE (Cat # L013V3).
Sandwich ELISA: MAD2L1-binding protein Antibody (4G11) [H00009587-M03] - Detection limit for recombinant GST tagged MAD2L1BP is 1 ng/ml as a capture antibody.
Species: Hu
Applications: ELISA, ICC/IF, S-ELISA, WB
Mitofusin 2
Antibody (4H8)
H00009927-M03
Western Blot: Mitofusin 2 Antibody (4H8) [H00009927-M03] - Analysis of MFN2 over-expressed 293 cell line, cotransfected with MFN2 Validated Chimera RNAi ( Cat # H00009927-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MFN2 monoclonal antibody (M03), clone 4H8 (Cat # H00009927-M03 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Immunohistochemistry-Paraffin: Mitofusin 2 Antibody (4H8) [H00009927-M03] - Analysis of monoclonal antibody to MFN2 on formalin-fixed paraffin-embedded human heart. Antibody concentration 5 ug/ml
Western Blot: Mitofusin 2 Antibody (4H8) [H00009927-M03] - Analysis of MFN2 expression in HeLa (Cat # L013V1).
(1 Review)
(23 Publications)
Species: Hu, Rt
Applications: ELISA, IHC, IHC-P, RNAi, WB
MBL Antibody
AF2307
MBL Antibody
(6 Publications)
Species: Hu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), WB
PRG2 Antibody
NBP3-04784
Western Blot: PRG2 Antibody [NBP3-04784] - Analysis of extracts of Mouse liver, using PRG2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry-Paraffin: PRG2 Antibody [NBP3-04784] - Human placenta using PRG2 Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunocytochemistry/Immunofluorescence: PRG2 Antibody [NBP3-04784] - Immunofluorescence analysis of C6 cells using PRG2 Rabbit pAb (NBP3-04784) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NF-L Antibody
NB300-131
Immunocytochemistry/Immunofluorescence: NF-L Antibody [NB300-131] - Analysis of mouse cerebellum section stained with rabbit pAb to NF-L, NB300-131, dilution 1:5,000 in red, and costained with chicken pAb to MBP, dilution 1:5,000, in green. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. NB300-131 antibody labels dendrites and axons of neuronal cells, and MBP antibody stains the network of myelin sheathes around axons.
Western Blot: NF-L Antibody [NB300-131] - Analysis of different tissue lysates using rabbit pAb to NF-L, NB300-131, dilution 1:20,000. in green. [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. The strong band at 68kDa corresponds to the NF-L protein.
Immunocytochemistry/Immunofluorescence: NF-L Antibody [NB300-131] - Neurofilament (NF-L antibody) used as axon marker in WT and KO mice using immunofluorescence staining to illustrate the axon morphology of retinal ganglion cells. Central part of retina is enlarged and displayed in WT and KO mice. Scale bars are 500 micrometers for left and 200 micrometers for right. Citation: Lin Y-S, Kuo K-T, Chen S-K, Huang H-S (2018) RBFOX3/NeuN is dispensable for visual function. PLoS ONE 13(2): e0192355. https://doi.org/10.1371/journal.pone.0192355
(8 Publications)
Species: Bv, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-P, KO, WB
GDAP1 Antibody
NBP1-84430
Immunocytochemistry/Immunofluorescence: GDAP1 Antibody [NBP1-84430] - Immunofluorescent staining of human cell line A549 shows localization to cytosol & mitochondria. Antibody staining shown in green.
Immunohistochemistry-Paraffin: GDAP1 Antibody [NBP1-84430] - Staining of human cerebral cortex shows high expression.
Immunohistochemistry-Paraffin: GDAP1 Antibody [NBP1-84430] - Staining of human pancreas shows low expression as expected.
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
EMP1
Overexpression Lysate
NBL1-10259
Western Blot: EMP1 Overexpression Lysate (Adult Normal) [NBL1-10259] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for EMP1.
Species: Hu
Applications: WB
Tetraspanin-4
Antibody
NBP1-59438
Western Blot: Tetraspanin-4 Antibody [NBP1-59438] - GST-Teraspanin EC2 Expressed in E. coli.
Western Blot: Tetraspanin-4 Antibody [NBP1-59438] - Jurkat cell lysate, concentration 0.2-1 ug/ml.
Immunohistochemistry: Tetraspanin-4 Antibody [NBP1-59438] - Human Adult Liver Observed Staining: Cytoplasm in hepatocytes Primary Antibody Concentration: 1 : 600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1 : 200 Magnification: 20X Exposure Time: 0.5 2.0 sec.
Species: Hu
Applications: IHC, WB
PRB4 Antibody
NBP3-05584
Western Blot: PRB4 Antibody [NBP3-05584] - Western blot analysis of extracts of various cell lines, using PRB4 antibody (NBP3-05584) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Species: Mu, Rt
Applications: WB
Uroplakin Ib
Antibody
NBP1-80656
Western Blot: Uroplakin Ib Antibody [NBP1-80656] - Analysis in control (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: Uroplakin Ib Antibody [NBP1-80656] - UPK 1b positive staining (brown) of murine bladder epithelium, 4% paraformaldehyde fixation, paraffin embedding, and pH 6 HEIR retrieval method and DAB kit used. IHC-P image of mouse submitted by a verified customer review.
Immunohistochemistry-Paraffin: Uroplakin Ib Antibody [NBP1-80656] - Staining of human urinary bladder shows strong cytoplasmic and membranous positivity in urothelial cells.
(1 Review)
Species: Hu, Mu
Applications: IHC, IHC-P, WB
Exosome component
10 Antibody
NBP1-32870
Western Blot: Exosome component 10 Antibody [NBP1-32870] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EXOSC10 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: Exosome component 10 Antibody [NBP1-32870] - Paraffin-embedded Cal27 xenograft, using antibody at 1:100 dilution.
Western Blot: Exosome component 10 Antibody [NBP1-32870] - Sample: 1000 ug 293T whole cell lysate/extract A. 30 ug 293T whole cell lysate/extract, B. Control with 2. 5 ug of preimmune rabbit IgG, C. Immunoprecipitation of EXOSC 10 protein by 2. 5 ug of EXOSC 10 antibody 7. 5% SDS-PAGE gel.
(2 Reviews)
(3 Publications)
Species: Ch, Hu
Applications: IHC, IHC-P, IP, KD, WB
PXMP2
Overexpression Lysate
NBL1-15012
PXMP2 Overexpression Lysate
Species: Hu
Applications: WB

Publications for PXMP2 Lysate (NBL1-15012) (0)

There are no publications for PXMP2 Lysate (NBL1-15012).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for PXMP2 Lysate (NBL1-15012) (0)

There are no reviews for PXMP2 Lysate (NBL1-15012). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for PXMP2 Lysate (NBL1-15012) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional PXMP2 Products

Array NBL1-15012

Bioinformatics Tool for PXMP2 Lysate (NBL1-15012)

Discover related pathways, diseases and genes to PXMP2 Lysate (NBL1-15012). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for PXMP2 Lysate (NBL1-15012)

Discover more about diseases related to PXMP2 Lysate (NBL1-15012).
 

Pathways for PXMP2 Lysate (NBL1-15012)

View related products by pathway.

PTMs for PXMP2 Lysate (NBL1-15012)

Learn more about PTMs related to PXMP2 Lysate (NBL1-15012).

Blogs on PXMP2

There are no specific blogs for PXMP2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Immunohistochemistry-Frozen: MBP Antibody (12) [NB600-717] - Staining of MBP in mouse spinal cord ventral white matter. Image from verified customer review.
MBP Antibody (12)
NB600-717
Western Blot: Mitofusin 2 Antibody (4H8) [H00009927-M03] - Analysis of MFN2 over-expressed 293 cell line, cotransfected with MFN2 Validated Chimera RNAi ( Cat # H00009927-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MFN2 monoclonal antibody (M03), clone 4H8 (Cat # H00009927-M03 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Mitofusin 2 Antibody (4H8)
H00009927-M03

Contact Information

Product PDFs

  PDF Datasheet
  Request CofA
  Request SDS

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our PXMP2 Overexpression Lysate and receive a gift card or discount.


Bioinformatics

Gene Symbol PXMP2