RAR beta/NR1B2 Antibody (336)

Images

 
Western Blot: Retinoic Acid Receptor beta Antibody (336) [NB200-323] - Western blot of Rat hippocampal lysate showing specific immunolabeling of the ~ 48k RAR-B isotype.

Product Details

Summary
Reactivity Hu, Rt, Mu, Ca, Gp, PmSpecies Glossary
Applications WB
Clone
336
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

RAR beta/NR1B2 Antibody (336) Summary

Immunogen
Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH. Accession # P10826
Specificity
Specific for endogenous levels of the ~48 kDa RAR beta/NR1B2 isotype.
Predicted Species
Mouse (100%), Guinea Pig (100%), Canine (100%), Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RARB
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
Application Notes
By Western blot analysis a ~48 kDa band can be seen using rat brain hippocampal homogenate. WB was tested in TBST (20 mM Tris/HCl (7.6), 137 mM NaCl, 1% Tween-20) containing 5% nonfat milk and 0.05% NaN3.
Theoretical MW
48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 3
using
NB200-323 in the following application:

Publications
Read Publication using
NB200-323 in the following applications:

  • WB
    1 publication

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 18272600). It is anticipated that the antibody will also work with canine, guinea pig, mouse and non-human primate tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for RAR beta/NR1B2 Antibody (336)

  • beta polypeptide
  • HAP
  • HBV-activated protein
  • NR1B2
  • RAR beta
  • RARB
  • retinoic acid receptor, beta
  • RRB2

Background

Retinoids can reverse various premalignant lesions as well as prevent some second primary tumors. The effects of retinoids are regulated by retinoic acid receptors (RAR) and retinoid X receptors, which act as ligand-activated transcription factors. Ligand-dependent transcriptional activation of RARs is a multistep process that ends with the formation of a multimeric co-activator complex on regulated promoters (1,2). Loss of RAR-beta expression and the accumulation of p53 and Ki67 proteins may help in the early identification of esophageal cancer in high-risk populations (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RAR beta/NR1B2 Antibody (NB200-323)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: WB.


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WB
(1)
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Reviews for RAR beta/NR1B2 Antibody (NB200-323) (2) 32

Average Rating: 3
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Other.

Reviews using NB200-323:
Filter by Applications
WB
(2)
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Human
(1)
Other
(1)
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Images Ratings Applications Species Date Details
Western Blot RAR beta/NR1B2 NB200-323
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3
reviewed by:
Verified Customer
WB Other 04/20/2015
View

Summary

ApplicationWestern Blot
Sample TestedOverexpressing COS cells
SpeciesOther
Lot336B
Western Blot RAR beta/NR1B2 NB200-323
Enlarge
3
reviewed by:
Verified Customer
WB Human 09/05/2014
View

Summary

ApplicationWestern Blot
Sample Tested293T and HePG2 cell lysate
SpeciesHuman

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for RAR beta/NR1B2 Antibody (NB200-323). (Showing 1 - 1 of 1 FAQ).

  1. I am planning to perform IHC staining of RARB on FFPE samples. I have found the reference "J Cutan Pathol 2009;36; 1141-1145" used an antibody from your company. Would you let me know any other references using the antibody for IHC staining of RARB?
    • Unfortunately we are not aware of any other publication wherein our Retinoic Acid Receptor beta antibodies were cited for their use in IHC application. From our sales records, it looks like the researcher used catalog number NB200-323 in the publication you mentioned. Retinoic Acid Receptor beta antibody NB200-323 is our best selling product among this category and it has also been cited for its use in WB application by Yang et al. in Am J Physiol Renal Physiol. 2008 Jun;294(6):F1433-40. We have at least 7 primary antibody products to Retinoic Acid Receptor beta that have been validated for IHC-P application.. We stand behind the quality of our products and offer 100% guarantee for the applications/species mentioned on the datasheets. We are always there to help if you come up with any difficulties and yes, we provide replacements/refunds after troubleshooting a problem, if you do not get your desired outcome! Therefore, please let us know if you have any specific questions or concerns on the IHC-P application of our Retinoic Acid Receptor beta antibodies. You may contact us at technical@novusbio.com.

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

3
5
0
4
0
3
2
2
0
1
0

Verified Customer
04/20/2015
Application: WB
Species: Other

Verified Customer
09/05/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol RARB
Entrez
Uniprot