RAR beta/NR1B2 Antibody (336)

Images

 
Western Blot: Retinoic Acid Receptor beta Antibody (336) [NB200-323] - Western blot of Rat hippocampal lysate showing specific immunolabeling of the ~ 48k RAR-B isotype.

Product Details

Summary
Reactivity Hu, Rt, Mu, Ca, GP, PmSpecies Glossary
Applications WB
Clone
336
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

RAR beta/NR1B2 Antibody (336) Summary

Immunogen
Peptide corresponding to amino acids from N-terminal region of human retinoic acid receptor, beta-isotype. The antigen is identical in both beta1 and beta2 isoforms.
Specificity
Specific for the ~48k RAR-beta isotype.
Predicted Species
Mouse (100%), Primate (100%), Canine (100%), Guinea Pig (100%). Backed by our 100% Guarantee.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RARB
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
Application Notes
By Western blot analysis a ~48 kDa band can be seen using rat brain hippocampal homogenate. WB was tested in TBST (20 mM Tris/HCl (7.6), 137 mM NaCl, 1% Tween-20) containing 5% nonfat milk and 0.05% NaN3.
Reviewed Applications
Read 2 Reviews rated 3
using
NB200-323 in the following application:

Publications
Read Publication using
NB200-323 in the following applications:

  • WB
    1 publication

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 18272600). It is anticipated that the antibody will also work with canine, guinea pig, mouse and non-human primate tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for RAR beta/NR1B2 Antibody (336)

  • beta polypeptide
  • HAP
  • HBV-activated protein
  • NR1B2
  • RAR beta
  • RARB
  • retinoic acid receptor, beta
  • RRB2

Background

Retinoids can reverse various premalignant lesions as well as prevent some second primary tumors. The effects of retinoids are regulated by retinoic acid receptors (RAR) and retinoid X receptors, which act as ligand-activated transcription factors. Ligand-dependent transcriptional activation of RARs is a multistep process that ends with the formation of a multimeric co-activator complex on regulated promoters (1,2). Loss of RAR-beta expression and the accumulation of p53 and Ki67 proteins may help in the early identification of esophageal cancer in high-risk populations (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB200-322
Species: Hu, Mu, Rt
Applications: WB, IHC-Fr
NBP2-47314
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-58922
Species: Hu
Applications: WB
NB200-106
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-03644
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NB100-91662
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
AF748
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
NBP2-37576
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC
AF4885
Species: Mu
Applications: WB, IP
NBP2-29463
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NB100-168
Species: Hu, Mu(-)
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready, Flow-IC
NBP2-16756
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-00637
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NB100-74413
Species: Hu, Mu, Rb
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP2-30083
Species: Hu, Mu
Applications: WB, ICC/IF, IHC-P
NBP2-15840
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB110-74569
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP

Publications for RAR beta/NR1B2 Antibody (NB200-323)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
All Applications
Filter By Species
Rat
(1)
All Species

Reviews for RAR beta/NR1B2 Antibody (NB200-323) (2) 32

Average Rating: 3
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Other.
We have 2 reviews tested in 1 application: WB.

Reviews using NB200-323:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot RAR beta/NR1B2 NB200-323
Enlarge
reviewed by:
Anonymous
WB Other 04/20/2015
View

Summary

ApplicationWestern Blot
Sample TestedOverexpressing COS cells
SpeciesOther
Lot336B

Blocking

Blocking DetailsBlocked in PBS with 5% BSA and 0.1% Tween-20, 1hr at RT.

Primary Anitbody

Dilution Ratio1:500 dilution in PBS with 5% BSA and 0.1% Tween-20. Incubated over night at 4C.

Secondary Antibody

Secondary DescriptionAnti-Mouse-HRP
Secondary Manufacturer Cat#Jackson-715-036-150
Secondary Concentration1:10000

Details

Detection NotesDetected with femto ECL system, 5 min exposure. COS cells overexpressing RARalpha2 and RARgamma2 served as negative controls.

Comments

CommentsNo cross-reactivity was detected towards RARalpha2 and RARgamma2.
Western Blot RAR beta/NR1B2 NB200-323
Enlarge
reviewed by:
Anonymous
WB Human 09/05/2014
View

Summary

ApplicationWestern Blot
Sample Tested293T and HePG2 cell lysate
SpeciesHuman
CommentsAS all other antibodies for RARbeta, this one works but the signal is faint, with a lot of background.

Blocking

Blocking Details5% milk in TBST buffer

Primary Anitbody

Dilution Ratio1:500, diluted in TBST buffer+5% milk

Secondary Antibody

Secondary Descriptiongoat anti-mouse IgG-HRP (SCBT)
Secondary Manufacturer Cat#sc-2031
Secondary Concentration1:5,000

Details

Detection NotesDetected using Santa Cruz Western Blotting Luminol Reagent, Exposure 2 min

Comments

CommentsAS all other antibodies for RARbeta, this one works but the signal is faint, with a lot of background.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for RAR beta/NR1B2 Antibody (NB200-323). (Showing 1 - 1 of 1 FAQ).

  1. I am planning to perform IHC staining of RARB on FFPE samples. I have found the reference "J Cutan Pathol 2009;36; 1141-1145" used an antibody from your company. Would you let me know any other references using the antibody for IHC staining of RARB?
    • Unfortunately we are not aware of any other publication wherein our Retinoic Acid Receptor beta antibodies were cited for their use in IHC application. From our sales records, it looks like the researcher used catalog number NB200-323 in the publication you mentioned. Retinoic Acid Receptor beta antibody NB200-323 is our best selling product among this category and it has also been cited for its use in WB application by Yang et al. in Am J Physiol Renal Physiol. 2008 Jun;294(6):F1433-40. We have at least 7 primary antibody products to Retinoic Acid Receptor beta that have been validated for IHC-P application and you may find the complete list here. We stand behind the quality of our products and offer 100% guarantee for the applications/species mentioned on the datasheets. We are always there to help if you come up with any difficulties and yes, we provide replacements/refunds after troubleshooting a problem, if you do not get your desired outcome! Therefore, please let us know if you have any specific questions or concerns on the IHC-P application of our Retinoic Acid Receptor beta antibodies. You may contact us at technical@novusbio.com.

Secondary Antibodies

 

Isotype Controls

Additional RAR beta/NR1B2 Products

Bioinformatics Tool for RAR beta/NR1B2 Antibody (NB200-323)

Discover related pathways, diseases and genes to RAR beta/NR1B2 Antibody (NB200-323). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for RAR beta/NR1B2 Antibody (NB200-323)

Discover more about diseases related to RAR beta/NR1B2 Antibody (NB200-323).
 

Pathways for RAR beta/NR1B2 Antibody (NB200-323)

View related products by pathway.

PTMs for RAR beta/NR1B2 Antibody (NB200-323)

Learn more about PTMs related to RAR beta/NR1B2 Antibody (NB200-323).
 

Research Areas for RAR beta/NR1B2 Antibody (NB200-323)

Find related products by research area.

Blogs on RAR beta/NR1B2

There are no specific blogs for RAR beta/NR1B2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

3
5
0
4
0
3
2
2
0
1
0

Anonymous
04/20/2015
Application: WB
Species: Other

Anonymous
09/05/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol RARB
Entrez
Uniprot