PUMA Antibody

Western Blot: PUMA Antibody [NBP1-52093] - (0.1ug/ml) staining of Peripheral Blood Lymphocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PUMA Antibody Summary

• Description: Novus Biologicals Goat PUMA Antibody (NBP1-52093) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. Anti-PUMA Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-DGGRPQRTVR corresponding to internal region according to NP_001120712.1.
• Epitope: C-DGGRPQRTVR
• Specificity: This antibody is expected to recognize isoform 1 (NP_001120712.1) only.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: BBC3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 24 kDa band observed in human peripheral blood mononucleocytes lysates (calculated MW of 26.5 kDa band according to NP_001120712.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PUMA Antibody

• BCL2 binding component 3
• bcl-2-binding component 3
• JFY1
• JFY-1
• p53 up-regulated modulator of apoptosis
• PUMAFLJ42994

Background

PUMA, (p53 upregulated modulator of apoptosis), is activated by p53 in cells undergoing p53-induced apoptosis (1-2). Localized in the mitochondria PUMA induces cytochrome c release and activates the rapid induction of programmed cell death. PUMA interacts with Bcl2 (3), Bcl-XL, Bcl2-like-1 protein and promotes mitochondrial translocation and multimerization of BAX. PUMA also plays a role in mediating p53-induced cell death through the cytochrome c/APAF1-dependent pathway (2). Two isoform of PUMA are known; PUMA-alpha and PUMA-beta.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PUMA Antibody (NBP1-52093)(3)

Publications using NBP1-52093

• Pagano C, Coppola L, Navarra G et al. N6-Isopentenyladenosine Impairs Mitochondrial Metabolism through Inhibition of EGFR Translocation on Mitochondria in Glioblastoma Cells Cancers 2022-12-08 [PMID: 36551529] (ICC/IF, Human)
• Kim J, Yu L, Chen W et al. Wild-Type p53 Promotes Cancer Metabolic Switch by Inducing PUMA-Dependent Suppression of Oxidative Phosphorylation Cancer Cell 2019-02-11 [PMID: 30712844] (WB, Human)
• Qiu W, Leibowitz B, Zhang L, Yu J. Growth factors protect intestinal stem cells from radiation-induced apoptosis by suppressing PUMA through the PI3K/AKT/p53 axis. Oncogene 2010-04-01 [PMID: 19966853]

Bioinformatics

• Gene Symbol: BBC3
• Entrez:
  • 27113(Human)
• Uniprot:
  • NP_001120712.1(Human)