PUM2 Antibody (740610) [Alexa Fluor® 405]

• Applications: WB
• Clone: 740610
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 405

PUM2 Antibody (740610) [Alexa Fluor® 405] Summary

• Immunogen: E. coli-derived recombinant human PUM2
  Pro103-Gly250
  Accession # Q8TB72
• Specificity: Detects human PUM2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human PUM1 is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

The Pumilio proteins PUM1 and PUM2 are RNA binding proteins that are repressors of mRNA translation. PUM2 is present in embryonic stem cells and adult germ cells. Both positive and negative influences of PUM2 on proliferation have been reported. Human PUM2 is a 1066 amino acid (aa), 114 kDa (predicted) protein with 6 potential Ser/Thr phosphorylation sites (aa 82, 136, 178, 182, 184, 587, and 672), an acetylation site (aa 672), and a pumilio domain (aa 706-1048). Within aa 103-250, human PUM2 shares 95% aa sequence identity with mouse and rat PUM2. A potential 987 aa isoform lacks aa 574-652.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.