PTP1B/PTPN1 Antibody

Western Blot: PTP1B/PTPN1 Antibody [NBP3-23194] - Western blot image of mouse SYF cSrc-transformed cells untreated (lanes 1 & 3) or treated (lanes 2 & 4) with pervanadate (1 mM for 30 min.). The blots were probed with rabbit polyclonal anti-PTP1B/PTPN1 (a.a. 146-157) (lanes 1 & 2) or anti-PTP1B/PTPN1 (Tyr-152) (lanes 3 & 4).

Immunocytochemistry/Immunofluorescence: PTP1B/PTPN1 Antibody [NBP3-23194] - Immunocytochemical labeling of PTP1B/PTPN1 in aldehyde-fixed and NP-40 permeabilized human NCI-H1915 lung carcinoma cells. The cells were labeled with rabbit polyclonal anti-PTP1B/PTPN1 (NBP3-23194) antibody. The antibody was detected using appropriate secondary antibodyconjugated to DyLight(R) 594.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

PTP1B/PTPN1 Antibody Summary

• Description: Stable for at least 1 year at -20°C.
• Immunogen: PTP1B/PTPN1 synthetic peptide (coupled to carrier protein) corresponds to amino acids from the N-terminal region of human PTP1B/PTPN1. This sequence has significant homology to the conserved site in rat and mouse PTP1B/PTPN1, and has homology to TC-PTP and MPTP, but not other protein tyrosine phosphatase family members. [UniProt# P18031]
• Specificity: The antibody detects a 50 kDa band corresponding to PTP1B/PTPN1 in western blots of human A431, Jurkat, HeLa, and mouse SYF cells. This reactivity can be specifically blocked using PTP1B/PTPN1 (a.a. 146-157) peptide.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PTPN1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS, 1 mg/ml BSA and 50% glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Alternate Names for PTP1B/PTPN1 Antibody

• EC 3.1.3.48
• protein tyrosine phosphatase 1B
• protein tyrosine phosphatase, non-receptor type 1
• Protein-tyrosine phosphatase 1B
• PTP1B
• PTP-1B
• PTP1Bprotein tyrosine phosphatase, placental
• PTPN1
• tyrosine-protein phosphatase non-receptor type 1

Background

The non-transmembrane protein tyrosine phosphatase, PTP1B, comprises 435 amino acids, of which the C-terminal 114 residues have been implicated in controlling both localization and function of this enzyme. Increased phosphorylation of PTP1B is seen to accompany the transition from G2 to M phase of the cell cycle. Immunofluorescence experiments indicate that PTP-1B is localized predominantly in the endoplasmic reticulum (ER). Subcellular fractionation is consistent with this localization and establishes that PTP-1B is tightly associated with microsomal membranes, with its phosphatase domain oriented towards the cytoplasm. The finding of a tyrosine phosphatase on the ER suggests new possibilities for cellular events controlled by tyrosine phosphorylation (2). It has also been shown that PTP1B is a novel substrate for Akt and that phosphorylation of PTP1B by Akt at Ser(50) may negatively modulate its phosphatase activity creating a positive feedback mechanism for insulin signaling (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTPN1