TC-PTP/PTPN2 Antibody (252294) [Unconjugated]

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and TS1 mouse helper T cell line. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human/Mouse/Rat TC‑PTP Monoclonal Antibody (Catalog # MAB1930) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, Recombinant Human PTP1B (Catalog # 1366-PT) and Recombinant Human TC‑PTP aa 2-314 (Catalog # 1930-PT) (1 ng/lane) were included. A specific band for TC-PTP was detected at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 252294
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

TC-PTP/PTPN2 Antibody (252294) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human TC-PTP
  Pro2-Asn314
  Accession # P17706
• Specificity: Detects human, mouse, and rat TC‑PTP in Western blots. In Western blots, no cross-reactivity with PTP1B is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: PTPN2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TC-PTP/PTPN2 Antibody (252294) [Unconjugated]

• protein tyrosine phosphatase, non-receptor type 2
• PTP2
• PTPN2
• PTPTPTN2
• T-cell protein tyrosine phosphatase
• T-cell protein-tyrosine phosphatase
• TCELLPTP
• TCPTP
• TC-PTP
• TCPTPEC 3.1.3.48
• tyrosine-protein phosphatase non-receptor type 2

Background

T-cell protein tyrosine phosphatase (TC-PTP), also known as PTPT and PTPN2, is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. This enzyme has two C-terminal end splice variants with distinctly different subcellular localizations. The shorter 45 kilodalton isoform is exclusively nuclear in resting cells, but redistrubutes to the cytosol upon stimulation with growth factors (1) and cellular stress (2). The longer 48 kilodalton isoform is exclusively found in the endoplasmic reticulum (3) and seems to have distinctly different physiologic substrates from the smaller isoform (1, 4). Although found in many cell types and tissues, TC-PTP is particularly prominent in hemopoietic cell types (5, 6). Knockout mice lacking TC-PTP are born viable but die 3 to 5 weeks after birth of erythropoietic and lymphopoietic deficits (7), indicating a critical role for TC-PTP in bone marrow maturation. TC-PTP will dephosphorylate a wide range of phosphoproteins, such as p52^Shc (6) and receptors for EGF (1), Insulin (8) and growth hormone (6). The recombinant protein lacks the C-terminal 100 amino acids that determine intracellular localization but is fully active (9).

1. Tiganis, T. et al. (1999) J. Biol. Chem. 274:27768.
2. Lam, M.H. et al. (2001) J. Biol. Chem. 276:37700.
3. Lorenzen, J.A. et al. (1995) J. Cell Biol. 131:631.
4. Tiganis, T. et al. (1998) Mol. Cell. Biol. 18:1622.
5. Cool, D.E. et al. (1989) Proc. Natl. Acad. Sci. USA 86:5257.
6. Pasquali, C. et al. (2003) Mol. Endocrinol. 17:2228.
7. You-Ten, K.E. et al. (1997) J. Exp. Med. 186:683.
8. Galic, S. et al. (2003) Mol. Cell. Biol. 23:2096.
9. Cool, D.E. et al. (1990) Proc. Natl. Acad. Sci. USA 87:7280.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TC-PTP/PTPN2 Antibody (MAB1930)(5)

Publications using MAB1930

• H Li, BS Hostager, T Arkee, GA Bishop Multiple mechanisms for TRAF3-mediated regulation of the T cell costimulatory receptor GITR The Journal of Biological Chemistry, 2021;0(0):101097. 2021-01-01 [PMID: 34418432] (Western Blot, Mouse)
• H Lee, M Kim, M Baek, LD Morales, IS Jang, TJ Slaga, J DiGiovanni, DJ Kim Targeted disruption of TC-PTP in the proliferative compartment augments STAT3 and AKT signaling and skin tumor development Sci Rep, 2017;7(0):45077. 2017-01-01 [PMID: 28322331] (Western Blot, Mouse)
• Zhou W, Jeyaraman K, Yusoff P, Shenolikar S Phosphorylation at tyrosine 262 promotes GADD34 protein turnover. J Biol Chem, 2013;288(46):33146-55. 2013-01-01 [PMID: 24092754] (Western Blot, Mouse)
• Moore F, Naamane N, Colli ML, Bouckenooghe T, Ortis F, Gurzov EN, Igoillo-Esteve M, Mathieu C, Bontempi G, Thykjaer T, Orntoft TF, Eizirik DL STAT1 is a master regulator of pancreatic {beta}-cell apoptosis and islet inflammation. J. Biol. Chem., 2011;286(2):929-41. 2011-01-01 [PMID: 20980260] (Western Blot, Rat)
• Borza CM, Chen X, Mathew S, Mont S, Sanders CR, Zent R, Pozzi A Integrin alpha1beta1 promotes caveolin-1 dephosphorylation by activating T cell protein-tyrosine phosphatase. J. Biol. Chem., 2010;285(51):40114-24. 2010-01-01 [PMID: 20940300] (Western Blot, Human)

Bioinformatics

• Gene Symbol: PTPN2
• Uniprot:
  • P17706()