Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and TS1 mouse helper T cell line. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human/Mouse/Rat TC‑PTP Monoclonal Antibody (Catalog # ...read more
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.5 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for TC-PTP/PTPN2 Antibody (252294) [Unconjugated]
protein tyrosine phosphatase, non-receptor type 2
T-cell protein tyrosine phosphatase
T-cell protein-tyrosine phosphatase
tyrosine-protein phosphatase non-receptor type 2
T-cell protein tyrosine phosphatase (TC-PTP), also known as PTPT and PTPN2, is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. This enzyme has two C-terminal end splice variants with distinctly different subcellular localizations. The shorter 45 kilodalton isoform is exclusively nuclear in resting cells, but redistrubutes to the cytosol upon stimulation with growth factors (1) and cellular stress (2). The longer 48 kilodalton isoform is exclusively found in the endoplasmic reticulum (3) and seems to have distinctly different physiologic substrates from the smaller isoform (1, 4). Although found in many cell types and tissues, TC-PTP is particularly prominent in hemopoietic cell types (5, 6). Knockout mice lacking TC-PTP are born viable but die 3 to 5 weeks after birth of erythropoietic and lymphopoietic deficits (7), indicating a critical role for TC-PTP in bone marrow maturation. TC-PTP will dephosphorylate a wide range of phosphoproteins, such as p52Shc (6) and receptors for EGF (1), Insulin (8) and growth hormone (6). The recombinant protein lacks the C-terminal 100 amino acids that determine intracellular localization but is fully active (9).
Tiganis, T. et al. (1999) J. Biol. Chem. 274:27768.
Lam, M.H. et al. (2001) J. Biol. Chem. 276:37700.
Lorenzen, J.A. et al. (1995) J. Cell Biol. 131:631.
Tiganis, T. et al. (1998) Mol. Cell. Biol. 18:1622.
Cool, D.E. et al. (1989) Proc. Natl. Acad. Sci. USA 86:5257.
Pasquali, C. et al. (2003) Mol. Endocrinol. 17:2228.
You-Ten, K.E. et al. (1997) J. Exp. Med. 186:683.
Galic, S. et al. (2003) Mol. Cell. Biol. 23:2096.
Cool, D.E. et al. (1990) Proc. Natl. Acad. Sci. USA 87:7280.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
Review this Product
Be the first to review our TC-PTP/PTPN2 Antibody (252294) [Unconjugated] and receive a gift card or discount.