TC-PTP/PTPN2 Antibody (252294) [Unconjugated]

Images

 
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and TS1 mouse helper T cell line. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human/Mouse/Rat TC‑PTP Monoclonal Antibody (Catalog # ...read more
Effects of TC-PTP deficiency on STAT3 dephosphorylation in keratinocytes following TPA treatment.Total cell lysates were resolved by SDS-PAGE and immunoblotted with antibodies specific for STAT3 and phosphorylated STAT3 ...read more
Effects of TC-PTP deficiency on STAT3 dephosphorylation in keratinocytes following TPA treatment.Total cell lysates were resolved by SDS-PAGE and immunoblotted with antibodies specific for STAT3 and phosphorylated STAT3 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clone
252294
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

TC-PTP/PTPN2 Antibody (252294) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human TC-PTP
Pro2-Asn314
Accession # P17706
Specificity
Detects human, mouse, and rat TC‑PTP in Western blots. In Western blots, no cross-reactivity with PTP1B is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
PTPN2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
Publications
Read Publications using
MAB1930 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TC-PTP/PTPN2 Antibody (252294) [Unconjugated]

  • protein tyrosine phosphatase, non-receptor type 2
  • PTP2
  • PTPN2
  • PTPTPTN2
  • T-cell protein tyrosine phosphatase
  • T-cell protein-tyrosine phosphatase
  • TCELLPTP
  • TCPTP
  • TC-PTP
  • TCPTPEC 3.1.3.48
  • tyrosine-protein phosphatase non-receptor type 2

Background

T-cell protein tyrosine phosphatase (TC-PTP), also known as PTPT and PTPN2, is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. This enzyme has two C-terminal end splice variants with distinctly different subcellular localizations. The shorter 45 kilodalton isoform is exclusively nuclear in resting cells, but redistrubutes to the cytosol upon stimulation with growth factors (1) and cellular stress (2). The longer 48 kilodalton isoform is exclusively found in the endoplasmic reticulum (3) and seems to have distinctly different physiologic substrates from the smaller isoform (1, 4). Although found in many cell types and tissues, TC-PTP is particularly prominent in hemopoietic cell types (5, 6). Knockout mice lacking TC-PTP are born viable but die 3 to 5 weeks after birth of erythropoietic and lymphopoietic deficits (7), indicating a critical role for TC-PTP in bone marrow maturation. TC-PTP will dephosphorylate a wide range of phosphoproteins, such as p52Shc (6) and receptors for EGF (1), Insulin (8) and growth hormone (6). The recombinant protein lacks the C-terminal 100 amino acids that determine intracellular localization but is fully active (9).

  1. Tiganis, T. et al. (1999) J. Biol. Chem. 274:27768.
  2. Lam, M.H. et al. (2001) J. Biol. Chem. 276:37700.
  3. Lorenzen, J.A. et al. (1995) J. Cell Biol. 131:631.
  4. Tiganis, T. et al. (1998) Mol. Cell. Biol. 18:1622.
  5. Cool, D.E. et al. (1989) Proc. Natl. Acad. Sci. USA 86:5257.
  6. Pasquali, C. et al. (2003) Mol. Endocrinol. 17:2228.
  7. You-Ten, K.E. et al. (1997) J. Exp. Med. 186:683.
  8. Galic, S. et al. (2003) Mol. Cell. Biol. 23:2096.
  9. Cool, D.E. et al. (1990) Proc. Natl. Acad. Sci. USA 87:7280.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TC-PTP/PTPN2 Antibody (MAB1930)(7)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 2 applications: Immunoprecipitation, Western Blot.


Filter By Application
Immunoprecipitation
(1)
Western Blot
(5)
All Applications
Filter By Species
Human
(1)
Mouse
(4)
Rat
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using MAB1930 Applications Species
Maitreyi S Joshi, Angel Stanoev, Jan Huebinger, Birga Soetje, Veronika Zorina, Lisaweta Ro beta mannek et al. The EGFR phosphatase RPTP gamma is a redox‐regulated suppressor of promigratory signaling The EMBO Journal 2023-05-15 [PMID: 36988334]
H Li, BS Hostager, T Arkee, GA Bishop Multiple mechanisms for TRAF3-mediated regulation of the T cell costimulatory receptor GITR The Journal of Biological Chemistry, 2021-08-18;0(0):101097. 2021-08-18 [PMID: 34418432] (Western Blot, Mouse) Western Blot Mouse
H Lee, M Kim, M Baek, LD Morales, IS Jang, TJ Slaga, J DiGiovanni, DJ Kim Targeted disruption of TC-PTP in the proliferative compartment augments STAT3 and AKT signaling and skin tumor development Sci Rep, 2017-03-21;7(0):45077. 2017-03-21 [PMID: 28322331] (Western Blot, Mouse) Western Blot Mouse
Chen X, Wang H, Liao H, Hu W, Gewin L, Mernaugh G, Zhang S, Zhang Z, Vega-Montoto L, Vanacore R, Fassler R, Zent R, Pozzi A Integrin-mediated type II TGF-beta receptor tyrosine dephosphorylation controls SMAD-dependent profibrotic signaling. J Clin Invest, 2014-07-01;124(8):3295-310. 2014-07-01 [PMID: 24983314] (Immunoprecipitation, Mouse) Immunoprecipitation Mouse
Zhou W, Jeyaraman K, Yusoff P, Shenolikar S Phosphorylation at tyrosine 262 promotes GADD34 protein turnover. J Biol Chem, 2013-10-03;288(46):33146-55. 2013-10-03 [PMID: 24092754] (Western Blot, Mouse) Western Blot Mouse
Moore F, Naamane N, Colli ML, Bouckenooghe T, Ortis F, Gurzov EN, Igoillo-Esteve M, Mathieu C, Bontempi G, Thykjaer T, Orntoft TF, Eizirik DL STAT1 is a master regulator of pancreatic {beta}-cell apoptosis and islet inflammation. J. Biol. Chem., 2010-10-27;286(2):929-41. 2010-10-27 [PMID: 20980260] (Western Blot, Rat) Western Blot Rat
Borza CM, Chen X, Mathew S, Mont S, Sanders CR, Zent R, Pozzi A Integrin alpha1beta1 promotes caveolin-1 dephosphorylation by activating T cell protein-tyrosine phosphatase. J. Biol. Chem., 2010-10-12;285(51):40114-24. 2010-10-12 [PMID: 20940300] (Western Blot, Human) Western Blot Human

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Bioinformatics

Gene Symbol PTPN2
Uniprot