PTEN Antibody [Unconjugated]

PTEN was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti- Human PTEN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6655) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

PTEN was detected in immersion fixed Neuro-2A mouse neuroblastoma cell line using Goat Anti-Human PTEN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6655) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

TIM-3 Association with Intracellular Kinases in CD8+/MART-1+ T cells.TIM-3 co-immunoprecipitation analysis of unactivated and 15 min. stimulation with anti-CD3/CD28 beads (activated). Equivalent amounts of protein (~2mg) were co-immunoprecipitated with pAb anti-TIM-3 antibody and western blot was performed using capillary electrophoresis. Cleared lysate served as a loading control for individual antibody reactivity. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0140694), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: IHC, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

PTEN Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human PTEN
  Thr2-Val403
  Accession # P60484
• Specificity: Detects human PTEN in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PTEN
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 1-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is covered by the following U.S. patent: USSN # 10/299,003.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PTEN Antibody [Unconjugated]

• BZS
• EC 3.1.3.16,10q23del
• EC 3.1.3.48
• EC 3.1.3.67
• GLM2
• MMAC1 phosphatase and tensin homolog deleted on chromosome 10
• MMAC1
• MMAC1MGC11227
• Mutated in multiple advanced cancers 1
• phosphatase and tensin homologDEC
• phosphatidylinositol-34,5-trisphosphate 3-phosphatase and dual-specificityprotein phosphatase PTEN
• PTEN
• PTEN1
• TEP1MHAM

Background

The tumor suppressor gene PTEN (phosphatase and tensin homolog deleted on chromosome 10), also known as MMAC1 (mutated in multiple advanced cancers 1), encodes a phosphatase that contains the catalytic signature motif (HCXXGXXRS/T) found in all members of the protein tyrosine phosphatase family. In vitro, the recombinant PTEN has both lipid phosphatase and protein phosphatase activities (1, 2). Interestingly, accumulating evidence has shown that the tumor suppressor activity of PTEN relies on its ability to dephosphorylate phosphatidylinositol (3, 4, 5)-triphosphate specifically at position 3 of the inositol ring (3). This activity reduces the levels of phosphatidylinositol (3, 4, 5)-triphosphate which is specifically produced from phosphatidylinositol (4, 5)-diphosphate by PI 3-kinase upon activation by a variety of stimuli. Therefore, PTEN antagonizes PI 3-kinase-induced downstream signaling events and cellular processes including cell growth, apoptosis and cell motility. In vivo, the importance of PTEN catalytic activity in its tumor suppressor functions is underscored by the fact that the majority of PTEN missense mutations detected in tumor specimens target the phosphatase domain and cause a loss in PTEN phosphatase activity (4).

1. Maehama, T. and J. Dixon (1998) J. Biol. Chem. 273:13375.
2. Das, S. et al. (2003) Proc. Natl. Acad. Sci. USA 100:7491.
3. Myers, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:13513.
4. Waite, K. and C. Eng (2002) Am. J. Hum. Genet. 70:829.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PTEN Antibody (AF6655)(2)

Publications using AF6655

• Xinyi Fan, Jeffrey Kraynak, Jonathan P.S. Knisely, Silvia C. Formenti, Wen H. Shen PTEN as a Guardian of the Genome: Pathways and Targets Cold Spring Harbor Perspectives in Medicine 2020-09-01 [PMID: 31932469]
• Tomkowicz B, Walsh E et al. TIM-3 Suppresses Anti-CD3/CD28-Induced TCR Activation and IL-2 Expression through the NFAT Signaling Pathway. PLoS One 2024-10-22 [PMID: 26492563] (Simple Western, Human)

Bioinformatics

• Gene Symbol: PTEN
• Uniprot:
  • P60484(Human)