After August 17, 2026, Novus Biologicals products and services will no longer be available on this website; you will access all products and services on rndsystems.com. Create your R&D Systems online account today.
HAF018). A specific band was detected for PSGL-1/CD162 monomer at approximately 110-120 and PSGL-1/CD162 homodimer at approximately 250-260 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
MAB0041, open histogram) followed by anti-Mouse IgG PE-conjugates secondary antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox" target="_blank">
VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox" target="_blank">
DY990). Sheep Anti-Human PSGL-1/CD162 Antigen Affinity-purified Polyclonal Antibody(Catalog # AF3345) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox" target="_blank">
HAF018). Specific bands were detected for PSGL‑1/CD162 at approximately 120, 250 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox" target="_blank">
HAF018). Specific bands were detected for PSGL1/ CD162 homodimer at approximately 250 kDa and PSGL1/ CD162 monomer at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B)." class="big_lightbox" target="_blank">
VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox" target="_blank">
CD162, also known as p-selectin glycoprotein ligand-1 (PSGL-1), is a 120 - 220 kDa, mucin-like type I transmembrane glycoprotein. CD162 binds to CD62P (P-Selectin), CD62E (E-Selectin) and CD62L (L-Selectin). The interactions between P-selectin and P-selectin glycoprotein ligand-1 (PSGL-1) mediate the earliest "rolling" of leukocytes on the lumenal surface of activated endothelium, and the interaction between leukocytes and activated platelets or other leukocytes found at sites of inflammation. CD162 is expressed on neutrophils, monocytes, and most lymphocytes including NK and T cells but PSGL-1 stains B cells at significantly lower levels than other cell types.
![Western Blot PSGL-1/CD162 Antibody (688124) [Unconjugated]](http://images.novusbio.com/images/antibody/PSGL1_MAB9962_Western_Blot_21827.jpg)
![Western Blot PSGL-1/CD162 Antibody (688101) [Unconjugated]](http://images.novusbio.com/images/antibody/PSGL1_MAB9961_Western_Blot_21538.jpg)
![Bioactivity PSGL-1/CD162 [Unconjugated]](http://images.novusbio.com/fullsize/protein/PSGL1_3345PS_3096.jpg)
![Flow Cytometry PSGL-1/CD162 Antibody (688124) [Unconjugated]](http://images.novusbio.com/images/antibody/PSGL1_MAB9962_Flow_Cytometry_23151.jpg)
![Immunocytochemistry PSGL-1/CD162 Antibody (688124) [Unconjugated]](http://images.novusbio.com/images/antibody/PSGL1_MAB9962_Immunocytochemistry__Immunofluorescence_21795.jpg)
![Immunohistochemistry PSGL-1/CD162 Antibody (688124) [Unconjugated]](http://images.novusbio.com/images/antibody/PSGL1_MAB9962_Immunohistochemistry_21796.jpg)
![ELISA PSGL-1/CD162 Antibody (688124) [Unconjugated]](http://images.novusbio.com/images/antibody/PSGL1_MAB9962_ELISA_23029.jpg)
![Western Blot PSGL-1/CD162 Antibody (688124) [Unconjugated]](http://images.novusbio.com/images/antibody/mab9962_human-psgl-1-cd162-mab-clone-688124-western-blot-2042022113146.jpg)
![Flow Cytometry PSGL-1/CD162 Antibody (688101) [Unconjugated]](http://images.novusbio.com/images/antibody/PSGL-1_MAB9961_Flow_Cytometry_11041.jpg)
![Immunocytochemistry PSGL-1/CD162 Antibody (688101) [Unconjugated]](http://images.novusbio.com/images/antibody/PSGL1_MAB9961_Immunocytochemistry__Immunofluorescence_21279.jpg)
![Immunohistochemistry PSGL-1/CD162 Antibody (688101) [Unconjugated]](http://images.novusbio.com/images/antibody/PSGL1_MAB9961_Immunohistochemistry_21278.jpg)
