Recombinant Human PSAP GST (N-Term) Protein

SDS-Page: Recombinant Human PSAP Protein [H00005660-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.

• Reactivity: Hu
• Applications: WB, ELISA, MA, PAGE, AP

Recombinant Human PSAP GST (N-Term) Protein Summary

• Description: A recombinant protein with GST tag at N-terminal corresponding to the amino acids 18-524 of Human PSAP
  
  

  Source: Wheat Germ (in vitro)

  Amino Acid Sequence: PVLGLKECTRGSAVWCQNVKTASDCGAVKHCLQTVWNKPTVKSLPCDICKDVVTAAGDMLKDNATEEEILVYLEKTCDWLPKPNMSASCKEIVDSYLPVILDIIKGEMSRPGEVCSALNLCESLQKHLAELNHQKQLESNKIPELDMTEVVAPFMANIPLLLYPQDGPRSKPQPKDNGDVCQDCIQMVTDIQTAVRTNSTFVQALVEHVKEECDRLGPGMADICKNYISQYSEIAIQMMMHMQPKEICALVGFCDEVKEMPMQTLVPAKVASKNVIPALELVEPIKKHEVPAKSDVYCEVCEFLVKEVTKLIDNNKTEKEILDAFDKMCSKLPKSLSEECQEVVDTYGSSILSILLEEVSPELVCSMLHLCSGTRLPALTVHVTQPKDGGFCEVCKKLVGYLDRNLEKNSTKQEILAALEKGCSFLPDPYQKQCDQFVAEYEPVLIEILVEVMDPSFVCLKIGACPSAHKPLLGTEKCIWGPSYWCQNTETAAQCNAVEHCKRHVWN

• Preparation Method: in vitro wheat germ expression system
• Details of Functionality: This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.
• Source: Wheat germ
• Protein/Peptide Type: Recombinant Protein
• Gene: PSAP
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoaffinity Purification
  • Protein Array
  • SDS-Page
  • Western Blot
• Theoretical MW: 81.29 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human PSAP GST (N-Term) Protein

• FLJ00245
• GLBA
• proactivator polypeptide
• prosaposin
• PSAP
• SAP1
• SAP1MGC110993
• sphingolipid activator protein-1

Background

PSAP( AAH01503, 1 a.a. - 525 a.a.) recombinant protein with GST.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for PSAP Recombinant Protein (H00005660-P01)(5)

Publications using H00005660-P01

• J Dietrich, M Roth, S König, G Geerling, S Mertsch, S Schrader Analysis of lacrimal gland derived mesenchymal stem cell secretome and its impact on epithelial cell survival Stem Cell Res, 2019-06-04;38(0):101477. 2019-06-04 [PMID: 31181482]
• Yamamoto-Tanaka M, Motoyama A, Miyai M et al. Mesotrypsin and Caspase-14 Participate in Prosaposin Processing - Potential Relevance to Epidermal Permeability Barrier Formation. J Biol Chem. 2014-05-28 [PMID: 24872419]
• Wu Y, Sun L, Zou W et al. Prosaposin, a regulator of estrogen receptor alpha, promotes breast cancer growth. Cancer Sci. 2012-08-10 [PMID: 22738294]
• Koochekpour, Shahriar ;Hu, Siyi. Biologically active recombinant human saposin C and PSAP. FreshPatents 2010-01-28
• Fan SM, Tsai CF, Yen CM et al. Inducing hair follicle neogenesis with secreted proteins enriched in embryonic skin. Biomaterials 2018-03-13 [PMID: 29567388]

Bioinformatics

• Gene Symbol: PSAP