PRPS1 Antibody

Images

 
Western Blot: PRPS1 Antibody [NBP1-31654] - Sample (30 ug of whole cell lysate) A: Molt-4 10% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: PRPS1 Antibody [NBP1-31654] - Analysis of methanol-fixed HeLa, at 1:200 dilution.
Immunohistochemistry-Paraffin: PRPS1 Antibody [NBP1-31654] - Paraffin-embedded SW480 xenograft, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, RM, XpSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

PRPS1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human PRPS1. The exact sequence is proprietary.
Predicted Species
Mouse (100%), Rat (100%), Xenopus (95%), Rhesus Macaque (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PRPS1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (95%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PRPS1 Antibody

  • CMTX5ARTS
  • deafness, X-linked 2, perceptive, congenital
  • DFN2
  • DFNX1KIAA0967
  • dJ1070B1.2 (phosphoribosyl pyrophosphate synthetase 1)
  • EC 2.7.6.1
  • Phosphoribosyl pyrophosphate synthase I
  • phosphoribosyl pyrophosphate synthetase 1
  • PPRibP
  • PRSI
  • PRS-I
  • ribose-phosphate diphosphokinase 1
  • ribose-phosphate pyrophosphokinase 1

Background

Phosphoribosylpyrophosphate synthetase (PRPS; EC 2.7.6.1) catalyzes the phosphoribosylation of ribose 5-phosphate to 5-phosphoribosyl-1-pyrophosphate, which is necessary for the de novo and salvage pathways of purine and pyrimidine biosynthesis (Roessler et al., 1990 [PubMed 2155397]). Three PRPS genes have been identified: the widely expressed PRPS1 and PRPS2 (MIM 311860) genes, which map to chromosome Xq22-q24 and Xp22, respectively, and PRPS3 (PRPS1L1; MIM 611566), which maps to chromosome 7 and appears to be transcribed only in testis (Becker, 2001).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Diseases for PRPS1 Antibody (NBP1-31654)

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Bioinformatics

Gene Symbol PRPS1
Entrez
Uniprot