PRPS2 Antibody - BSA Free

Western Blot: PRPS2 Antibody [NBP1-31435] - Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE PRPS2 antibody, antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: PRPS2 Antibody [NBP1-31435] - Analysis of HaLa, using NBP1-31435 at 1:200 dilution.

Immunohistochemistry: PRPS2 Antibody [NBP1-31435] - Representative immunohistochemistry images of PRPS2 protein expression from the whole TMA with low intensity (b1) in normal prostate tissue (K11, scale bar = 0.2 mm), with low intensity (b4) in prostate cancer tissue (A9, scale bar = 0.2 mm), with intermediate intensity (b3) in prostate cancer tissue (G5, scale bar = 0.2 mm), and with high intensity (b2) in prostate cancer tissue (D2, scale bar = 0.2 mm). The percentage of PRPS2 positive cells in D2 is 90%, in G5 is 70%, and in A9 is 15%. Therefore, the percentage score of the case in D7 is 3 and its total protein expression score is 3 x 3 = 9. The percentage score of the case in H7 is 2 and its total protein expression score is 2 x 2 = 4. The percentage score of the case in E4 is 1 and its total protein expression score is 1 x 1 = 1. Image collected and cropped by CiteAb from the following publication (jcancer.org/v11p1027.htm), licensed under a CC-BY license.

Immunohistochemistry-Paraffin: PRPS2 Antibody [NBP1-31435] - Paraffin-embedded Hep3B xenograft, using antibody at 1:100 dilution.

Western Blot: PRPS2 Antibody [NBP1-31435] - The effects of PRPS2 knockdown confirmed by qRT-PCR (top) and Western blotting (bottom). Image collected and cropped by CiteAb from the following publication (jcancer.org/v11p1027.htm), licensed under a CC-BY license.

Western Blot: PRPS2 Antibody [NBP1-31435] - Knockdown of PRPS2 decreased prostate cancer cell growth. (A) The effects of PRPS2 knockdown confirmed by qRT-PCR (top) & Western blotting (bottom). (B) In the CCK‑8 assay, cell viability was decreased in si‑PRPS2 compared with NC. (C) In the plate colony formation assay, colony formation was obviously decreased in si-PRPS2 cells transfected. ***P < 0.001. NC: negative controls. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31956349), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: PRPS2 Antibody [NBP1-31435] - Knockdown of PRPS2 inhibits xenograft tumor growth in vivo. (A) PRPS2 shRNA effectively blocked its protein expression in PC-3 cells. Cells were stably transfected with scramble (sh-Ctrl) or PRPS2 shRNA (sh-PRPS2). PRPS2 protein levels were normalized to alpha -Tubulin. (B) Gross observation of xenograft tumor size in NOD/SCID mice. (C & D) Silencing of PRPS2 inhibited the tumor growth, including tumor volume (P < 0.001) & weight (P = 0.028, n = 6). (E) H&E‑stained paraffin‑embedded sections obtained from xenografts of PC-3 cells. (F) Top: Immunohistochemical analysis of Ki-67 in the xenografts; Bottom: The apoptosis in tumor tissues was evaluated by TUNEL assay (×200). Graphical illustrated the quantification of Ki-67 & TUNEL positive cells percentage. ***P < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31956349), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: PRPS2 Antibody [NBP1-31435] - PRPS2 expression in prostate cancer tissues & prostate cancer cell lines. (A) Representative immunohistochemistry images of PRPS2 protein expression from the whole TMA with low intensity (b1) in normal prostate tissue (K11, scale bar = 0.2 mm), with low intensity (b4) in prostate cancer tissue (A9, scale bar = 0.2 mm), with intermediate intensity (b3) in prostate cancer tissue (G5, scale bar = 0.2 mm), & with high intensity (b2) in prostate cancer tissue (D2, scale bar = 0.2 mm). The percentage of PRPS2 positive cells in D2 is 90%, in G5 is 70%, & in A9 is 15%. Therefore, the percentage score of the case in D7 is 3 & its total protein expression score is 3 × 3 = 9. The percentage score of the case in H7 is 2 & its total protein expression score is 2 × 2 = 4. The percentage score of the case in E4 is 1 & its total protein expression score is 1 × 1 = 1. (B) Western blotting & real-time qPCR analysis of PRPS2 protein & mRNA expression in prostate cancer cell lines & normal prostate epithelial cell lines (RWPE-1). (*P < 0.05; **P < 0.01; ***P < 0.001). PRPS2: Phosphoribosyl pyrophosphate synthetase 2; TMA: tissue microarray. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31956349), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ch, Pm, Xp
• Applications: WB, ICC/IF, IHC, Mycoplasma
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PRPS2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PRPS2 Antibody - BSA Free (NBP1-31435) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-PRPS2 Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human PRPS2. The exact sequence is proprietary.
• Predicted Species: Mouse (97%), Rat (97%), Porcine (97%), Chimpanzee (98%), Bovine (97%), Chicken (97%), Xenopus (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRPS2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockdown Validated
  • Western Blot 1:500-1:3000
• Theoretical MW: 35 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (96%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for PRPS2 Antibody - BSA Free

• EC 2.7.6.1
• Phosphoribosyl pyrophosphate synthase II
• phosphoribosyl pyrophosphate synthetase 2
• PPRibP synthetase
• PPRibP
• PRSII
• PRS-II
• ribose-phosphate diphosphokinase 2
• ribose-phosphate pyrophosphokinase 2

Background

PRPS2 catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 of both cytosolic and mitochondrial tRNAs, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

&#9888; WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for PRPS2 Antibody (NBP1-31435)(4)

Publications using NBP1-31435

• Emmanuel Benichou, Bolaji Seffou, Selin Topçu, Ophélie Renoult, Véronique Lenoir, Julien Planchais, Caroline Bonner, Catherine Postic, Carina Prip-Buus, Claire Pecqueur, Sandra Guilmeau, Marie-Clotilde Alves-Guerra, Renaud Dentin The transcription factor ChREBP Orchestrates liver carcinogenesis by coordinating the PI3K/AKT signaling and cancer metabolism Nature Communications 2024-02-29 [PMID: 38424041]
• Chen L, Zhou Q, Zhang P et al. Direct stimulation of de novo nucleotide synthesis by O-GlcNAcylation Nature chemical biology 2023-06-12 [PMID: 37308732] (WB, Human)
• Liu G, Luo Y, Hou P PRPS2 Enhances Resistance to Cisplatin via Facilitating Exosomes-mediated Macrophage M2 Polarization in Non-small Cell Lung Cancer Immunological investigations 2021-07-12 [PMID: 34251965]
• Qiao H, Tan X, Lv D, et al. Phosphoribosyl pyrophosphate synthetases 2 knockdown inhibits prostate cancer progression by suppressing cell cycle and inducing cell apoptosis J. Cancer 2019-12-13 [PMID: 31956349] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: PRPS2