Prostatic Acid Phosphatase/ACPP Antibody (ACPP/8409R)

Formalin-fixed, paraffin-embedded human prostate carcinoma stained with Prostatic Acid Phosphatase/ACPP antibody (ACPP/8409R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

• Reactivity: Hu
• Applications: IHC
• Clone: ACPP/8409R
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Prostatic Acid Phosphatase/ACPP Antibody (ACPP/8409R) Summary

• Description: Positive Controls: PC12 cells. Normal prostate or prostate carcinoma tissue.
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment (around aa200-400) of human Prostatic Acid Phosphatase/ACPP (exact sequence is proprietary)
• Localization: Cytoplasm.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ACP3
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  
  Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 10mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Prostatic Acid Phosphatase/ACPP Antibody (ACPP/8409R)

• acid phosphatase, prostate
• ACP3
• ACP-3
• ACPP
• EC 3.1.3.2
• PAP
• Prostatic Acid Phosphatase
• prostatic acid phosphotase

Background

Prostatic Acid Phosphatase encodes an enzyme which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is synthesized under androgen regulation and secreted by the epithelial cells of the prostrate gland.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACP3