Proinsulin Antibody (CCI-17) - BSA Free

• Reactivity: Hu, Rt
• Applications: WB, ELISA
• Clone: CCI-17
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Proinsulin Antibody (CCI-17) - BSA Free Summary

• Immunogen: Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with fragment of rat proinsulin conjugated with a carrier protein.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: INS
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Sandwich ELISA 1:100-1:2000
  • Western Blot
• Application Notes: Use in Western blot reported in reported in scientific literature (PMID: 31311313). Binds specifically to proinsulin and does not bind to insulin or C-peptide. For sandwich immunoassay it is recommended to use MAbs in pairs with MAbs specific to N-terminal part of C-peptides I and II. Recommended pair for sandwich-type rat proinsulin I and II immunoassay is (capture-detection): NB100-73013 - NBP1-05442.

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 31311313).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Alternate Names for Proinsulin Antibody (CCI-17) - BSA Free

• IDDM2
• ILPR
• insulin
• IRDN
• MODY10
• Proinsulin

Background

A precursor of insulin, with a molecular weight of 8,000 to 10,000. It has minimum hormonal activity and is converted to insulin by removal of the connecting C peptide, leaving the two (A and B) chains. From every molecule of proinsulin, one molecule of insulin plus one molecule of C Peptide are produced.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Proinsulin Antibody (NB100-73013)(11)

Publications using NB100-73013

• Liu Y, Yang X, Zhou J et Al. OSGEP regulates islet ?-cell function by modulating proinsulin translation and maintaining ER stress homeostasis in mice Nat Commun 2024-12-02 [PMID: 39622811]
• Arunagiri A, Alam M, Haataja L, Draz H et Al. Proinsulin folding and trafficking defects trigger a common pathological disturbance of endoplasmic reticulum homeostasis Protein Sci 2024-03-21 [PMID: 38511500]
• Xu X, Arunagiri A, Alam M, Haataja L et Al. Nutrient-dependent regulation of ?-cell proinsulin content J Biol Chem 2023-05-20 [PMID: 37209827]
• Xu X, Arunagiri A, Haataja L et al. Proteasomal degradation of wild-type proinsulin in pancreatic beta cells The Journal of biological chemistry 2022-08-18 [PMID: 35988641] (ICC/IF, Rat)
• Chen CW, Guan BJ, Alzahrani MR et al. Adaptation to chronic ER stress enforces pancreatic beta-cell plasticity Nature communications 2022-08-08 [PMID: 35941159] (WB, ICC/IF)
  
  Details:
  Dilutions: 1:1000
• Miyake M, Sobajima M, Kurahashi K, Shigenaga A et Al. Identification of an endoplasmic reticulum proteostasis modulator that enhances insulin production in pancreatic ? cells Cell Chem Biol 2022-02-10 [PMID: 35143772]
• Xu X, Huang Y, Li X et al. The Role of TRAP gamma/SSR3 in Preproinsulin Translocation into the Endoplasmic Reticulum Diabetes 2021-12-02 [PMID: 34857543]
• Herlea-Pana O, Eeda V, Undi RB Et al. Pharmacological Inhibition of Inositol-Requiring Enzyme 1 alpha RNase Activity Protects Pancreatic Beta Cell and Improves Diabetic Condition in Insulin Mutation-Induced Diabetes Frontiers in endocrinology 2021-10-05 [PMID: 34675883] (WB, Mouse)
• Chen YJ, Knupp J, Arunagiri A Et al. PGRMC1 acts as a size-selective cargo receptor to drive ER-phagic clearance of mutant prohormones Nature communications 2021-10-13 [PMID: 34645803] (WB)
• Huang Y, Xu X, Arvan P, Liu M Deficient endoplasmic reticulum translocon-associated protein complex limits the biosynthesis of proinsulin and insulin FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2021-05-01 [PMID: 33811688]
• Liu S, Li X, Yang J et al. Misfolded proinsulin impairs processing of precursor of insulin receptor and insulin signaling in beta cells FASEB J. 2019-07-16 [PMID: 31311313] (WB, Human)

Review for Proinsulin Antibody (NB100-73013) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: cesar barrabi WB Rat 06/05/2019

Summary

• Application: Western Blot
• Sample Tested: Rat pancreatic beta cell line
• Species: Rat
• Lot: CCI-17

Bioinformatics

• Gene Symbol: INS
• Entrez:
  • 24505(Rat)