Proinsulin Antibody (253627)

Images

 
Proinsulin was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using Mouse Anti-Human/Mouse Proinsulin Monoclonal Antibody (Catalog # MAB13361) at 10 µg/mL for 3 hours at room temperature. ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications CyTOF-ready, Flow, ICC/IF
Clone
253627
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Proinsulin Antibody (253627) Summary

Immunogen
E. coli-derived recombinant human Proinsulin
Phe25-Asn110
Accession # P01308
Specificity
Detects human and mouse Proinsulin. Does not detect mature insulin.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
INS
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
Publications
Read Publications using
MAB13361 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Proinsulin Antibody (253627)

  • IDDM2
  • ILPR
  • insulin
  • IRDN
  • MODY10
  • Proinsulin

Background

Proinsulin is synthesized as a single chain, 110 amino acid (aa) preproprecursor that contains a 24 aa signal sequence and an 86 aa proinsulin propeptide. Following removal of the signal peptide, the proinsulin peptide undergoes further proteolysis to generate mature insulin, a 51 aa disulfide-linked dimer that consists of a 30 aa B chain (aa 25‑54) bound to a 21 aa A chain (aa 90‑110). The 34 aa intervening peptide (aa 55‑89) that connects the B and A chains is termed the C-peptide. Human proinsulin shares 84% and 80% aa sequence identity with rat and bovine proinsulin, respectively. Most of the sequence variation between species occurs in the region of the C-peptide (1). This peptide generates a structural conformation that allows for the correct formation of the intrachain disulphide bonds (1). Insulin is a molecule that facilitates the cellular uptake of glucose. This is accomplished by regulating the appearance of membrane glucose transporters. Low insulin levels or lack of insulin are associated with type 2 and type 1 diabetes mellitus, respectively. These conditions are associated with an increased risk for microvascular complications such as retinopathy, nephropathy, and peripheral neuropathy (3). Proinsulin also circulates, but its physiologic role is less well understood. It does possess about 25% of the activity of mature insulin, but it would seem unlikely to be a natural substitute for insulin (4). In type 2 diabetes, an elevated proinsulin to insulin ratio in the circulation is a well-known abnormality (5‑9). Perhaps this abnormality represents either compromised proteolytic processing or a general inability to process increased levels of insulin precursor (5). In any event, proinsulin will stimulate amylin secretion by beta -cells, and amyloid formation in pancreatic islets that promotes decreased beta cell function (10). Studies also suggest that fasting serum proinsulin may be a better predictor of future type 2 diabetes than fasting insulin levels in obese children (11).

  1. Bell, G.I. et al. (1980) Nature 284:26.
  2. Barbetti, F. et al. (1990) J. Clin. Endocrinol. Metab. 71:164.
  3. Forst, T. et al. (2008) Exp. Diabetes Res. 2008:176245.
  4. Steffes, M.W. et al. (2003) Diabetes Care 26:832.
  5. Roder, M.E. et al. (1999) Diabetes Care 22:609.
  6. Porte, D. Jr. (1991) Diabetes 40:166.
  7. Gordon, P. et al. (1974) Diabetologia 34:483.
  8. Saad, M.F. et al. (1990) J. Clin. Endocrinol. Metab. 70:1247.
  9. Roder, M.E. et al. (1995) J. Clin. Endocrinol. Metab. 80:2359.
  10. Dworacka, M. et al. (2006) Int. J. Clin. Pharmacol. Ther. 44:14.
  11. Kamoda, T. et al. (2006) Diabetes Obes. Metab. 8:192.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Proinsulin Antibody (MAB13361)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: Flow Cytometry, ICC, IHC-Fr.


Filter By Application
Flow Cytometry
(3)
ICC
(1)
IHC-Fr
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(3)
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Showing Publications 1 - 6 of 6.
Publications using MAB13361 Applications Species
J Lee, K Kim, JH Cho, JY Bae, TP O'Leary, JD Johnson, YC Bae, EK Kim Insulin synthesized in the paraventricular nucleus of the hypothalamus regulates pituitary growth hormone production JCI Insight, 2020;0(0):. 2020 [PMID: 32644973] (ICC, Mouse) ICC Mouse
M Sambataro, L Sambado, E Trevisiol, M Cacciatore, A Furlan, PM Stefani, E Seganfredd, E Durante, S Conte, S Della Bell, A Paccagnell, AP Dei Tos Proinsulin-expressing dendritic cells in type 2 neuropathic diabetic patients with and without foot lesions FASEB J., 2018;0(0):fj201701279RR. 2018 [PMID: 29436863] (Flow Cytometry, Human) Flow Cytometry Human
A TLR9 agonist promotes IL-22-dependent pancreatic islet allograft survival in type 1 diabetic mice Nat Commun, 2016;7(0):13896. 2016 [PMID: 27982034] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Jonatan D, Spence J, Method A, Kofron M, Sinagoga K, Haataja L, Arvan P, Deutsch G, Wells J Sox17 regulates insulin secretion in the normal and pathologic mouse beta cell. PLoS ONE, 2014;9(8):e104675. 2014 [PMID: 25144761] (IHC-Fr, Mouse) IHC-Fr Mouse
Cao M, Mao Z, Kam C, Xiao N, Cao X, Shen C, Cheng K, Xu A, Lee K, Jiang L, Xia J PICK1 and ICA69 control insulin granule trafficking and their deficiencies lead to impaired glucose tolerance. PLoS Biol, 2013;11(4):e1001541. 2013 [PMID: 23630453]
Chen W, Begum S, Opare-Addo L Promotion of beta-cell differentiation in pancreatic precursor cells by adult islet cells. Endocrinology, 2009;150(2):570-9. 2009 [PMID: 18845629] (Flow Cytometry, Human) Flow Cytometry Human

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 IC13361U
Alexa Fluor 405 IC13361V
Alexa Fluor 488 IC13361G
Alexa Fluor 594 IC13361T
Alexa Fluor 647 IC13361R
Alexa Fluor 700 IC13361N
Alexa Fluor 750 IC13361S
Biotin BAM13361

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Bioinformatics

Gene Symbol INS
Uniprot