Prohibitin Antibody - BSA Free

Western Blot: Prohibitin Antibody [NBP2-19944] - A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat2 whole cell lysate/extract12 % SDS-PAGE Prohibitin antibody dilution: 1:1000

Immunocytochemistry/Immunofluorescence: Prohibitin Antibody [NBP2-19944] - SKNSH cells were fixed in 2% paraformaldehyde/culture medium at 37 c for 30 min.Green: Prohibitin protein stained by Prohibitin antibody diluted at 1:1000.Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: Prohibitin Antibody [NBP2-19944] - Paraffin-embedded mouse liver. Prohibitin antibody dilution: 1:500.

Western Blot: Prohibitin Antibody [NBP2-19944] - Sample (30 ug of whole cell lysate) A: IMR32 B: U87-MG 12% SDS PAGE gel, diluted at 1:1000.

Western Blot: Prohibitin Antibody [NBP2-19944] - A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug GL261 whole cell lysate/extractC. 30 ug C8D30 whole cell lysate/extract 12 % SDS-PAGE Prohibitin antibody dilution: 1:1000

Immunohistochemistry-Paraffin: Prohibitin Antibody [NBP2-19944] - Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: Prohibitin Antibody [NBP2-19944] - Paraffin-embedded mouse intestine. Prohibitin antibody dilution: 1:500.

• Reactivity: Hu, Mu, Rt, Bv, Ca, Ch, Fe, Pm, RM, Ze
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Prohibitin Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Prohibitin. The exact sequence is proprietary.
• Localization: Mitochondrion inner membrane
• Marker: Mitochondria Marker
• Predicted Species: Primate (100%), Chicken (96%), Canine (100%), Rhesus Macaque (100%), Zebrafish (94%), Feline (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PHB1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 30 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chimpanzee (100%), Cat (100%), Xenopus laevis (89%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Prohibitin Antibody - BSA Free

• PHB
• PHB1
• Prohibitin

Background

Prohibitin is an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: PHB1
• Uniprot:
  • P35232()