Progranulin/PGRN Antibody (333731)

Progranulin was detected in perfusion fixed frozen sections of mouse kidney using Rat Anti-Mouse Progranulin Monoclonal Antibody (Catalog # MAB25571) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, IHC
• Clone: 333731
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Progranulin/PGRN Antibody (333731) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Progranulin/PGRN
  Thr18-Leu589
  Accession # P28798
• Specificity: Detects mouse Progranulin/PGRN in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant human Progranulin is observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: Grn
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Progranulin/PGRN Antibody (333731)

• Acrogranin
• CLN11
• GEP
• GP88
• granulin precursor
• GRN
• PCDGF
• PEPI
• PGRN
• Proepithelin
• Progranulin

Background

Progranulin, also known as acrogranin, PC cell-derived growth factor (PCDGF) and epithelin/granulin precursor, is a ubiquitously expressed, 88 kDa, secreted glycoprotein (1‑3). Structurally, it belongs to none of the well-established growth factor families (4). Mouse Progranulin is 589 amino acids (aa) in length and contains a 17 aa signal sequence and a 572 aa mature region that has four potential sites for N-linked glycosylation. It has a highly repetitive organization, containing seven tandem copies of a 55‑57 aa consensus motif that contains 12 conserved cysteine residues: VXCX_5-6CX₅CCX₈CCX₆CCXDX₂HCCPX₄CX_5-6CX₂ (1). Progranulin is secreted in an intact form (2, 4) or undergoes proteolysis leading to the release of multiple peptides made from the seven tandem repeats, the granulins (5‑7). Mouse Progranulin shares 87% and 75% aa sequence identity with rat and human Progranulin, respectively. Progranulin is involved in the regulation of cellular proliferation, as well as differentiation, development, and pathological processes (4). It has been isolated as a differentially expressed gene during mesothelial differentiation (8), macrophage development (9), development synovium of rheumatoid arthritis and osteoarthritis (10), sexual differentiation of the brain (11), and has also been shown to be a mediator of cartilage proliferation plus of wound response and tissue repair (4, 12‑13). High levels of Progranulin expression have been found to be associated with several human cancers, and are believed to contribute to tumorigenesis in breast cancer, clear cell renal carcinoma, invasive ovarian carcinoma, glioblastoma, adipocyte teratoma, and multiple myeloma (4, 5, 12, 14‑20).

1. Plowman, G.D. et al. (1992) J. Biol. Chem. 267:13073. 
2. Zhou, J. et al. (1993) J. Biol. Chem. 268:10863. 
3. Liu, Y. et al. (2007) BMC Cancer 7:22. 
4. Xu, K. et al. (2007) J. Biol. Chem. 282:11347. 
5. Davidson, B. et al. (2004) Cancer 100:2139. 
6. Zanocco-Marani, T. et al. (1999) Cancer Res. 59:5331. 
7. Lu, R. and G. Serrero (2000) Proc. Natl. Acad. Sci. U.S.A. 97:3993. 
8. Sun, X. et al. (2004) Am. J. Respir. Cell Mol. Biol. 30:510. 
9. Barreda, D.R. et al. (2004) Dev. Comp. Immunol. 28:727 
10. Justen, H.P. et al. (2000) Mol. Cell Biol. Res. Commun. 3:165.
11. Suzuki, M. and M. Nishiahara (2002) Mol. Genet. Metab. 75:31.
12. He, Z. et al. (2003) Nat. Med. 9:225.
13. Zhu, J. et al. (2002) Cell 111:867.
14. He, Z. and A. Bateman (2003) J. Mol. Med. 81:600.
15. Bateman, A. et al. (1990) Biochem. Biophys. Res. Commun. 173:1161.
16. Gonzalez, E.M. et al. (2003) J. Biol. Chem. 278:38113.
17. Jones, M.B. et al. (2003) Gynecol. Oncol. 88:S136.
18. Wang, W. et al. (2003) Clin. Cancer Res. 9:2221.
19. Zhang, H. and G. Serrero (1998) Proc. Natl. Acad. Sci. U.S.A. 95:14202.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Progranulin/PGRN Antibody (MAB25571)(4)

Publications using MAB25571

• J Liu, X Lai, R Yu, H Ding, H Bai, Z Yang, Y Yin, F Xu, J Cao Progranulin aggravates lethal Candida albicans sepsis by regulating inflammatory response and antifungal immunity PloS Pathogens, 2022-09-19;18(9):e1010873. 2022-09-19 [PMID: 36121866] (In Vivo, Mouse)
• X Wang, J Yuan, H Wang, N Gan, Q Zhang, B Liu, J Wang, Z Shu, L Rao, X Gou, H Zhang, Y Yin, X Zhang Progranulin Decreases Susceptibility to Streptococcus pneumoniae in Influenza and Protects against Lethal Coinfection J. Immunol., 2019-09-13;0(0):. 2019-09-13 [PMID: 31519865] (IHC-P, Mouse)
• V Quaranta, C Rainer, SR Nielsen, ML Raymant, MS Ahmed, DD Engle, A Taylor, T Murray, F Campbell, DH Palmer, DA Tuveson, A Mielgo, MC Schmid Macrophage-derived granulin drives resistance to immune checkpoint inhibition in metastatic pancreatic cancer Cancer Res., 2018-05-22;0(0):. 5/22/2018 [PMID: 29789416]
• Elkabets M, Gifford AM, Scheel C, Nilsson B, Reinhardt F, Bray MA, Carpenter AE, Jirstrom K, Magnusson K, Ebert BL, Ponten F, Weinberg RA, McAllister SS Human tumors instigate granulin-expressing hematopoietic cells that promote malignancy by activating stromal fibroblasts in mice. J. Clin. Invest., 2011-01-25;121(2):784-99. 2011-01-25 [PMID: 21266779] (ELISA Development, Mouse)

Bioinformatics

• Gene Symbol: Grn
• Uniprot:
  • P28798(Mouse)