Profilin 1 Antibody (S06-6H7)

Western Blot: Profilin 1 Antibody (S06-6H7) [NBP3-19483] - Western blot detection of Profilin 1 in C6, 3T3, Hela cell lysates using NBP3-19483 (1:1000 diluted). Predicted band size: 15kDa. Observed band size: 15kDa.

Immunohistochemistry-Paraffin: Profilin 1 Antibody (S06-6H7) [NBP3-19483] - Immunohistochemistry of Profilin 1 in paraffin-embedded Human tonsil using NBP3-19483 at dilution 1/100

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: S06-6H7
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.3 mg/ml

Profilin 1 Antibody (S06-6H7) Summary

• Description: Novus Biologicals Rabbit Profilin 1 Antibody (S06-6H7) (NBP3-19483) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human Profilin 1 (Uniprot # P07737)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PFN1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1/50
  • Immunohistochemistry-Paraffin
  • Western Blot 1/2000
• Theoretical MW: 15 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Affinity purified

Alternate Names for Profilin 1 Antibody (S06-6H7)

• PFN1
• PROF1
• Profilin 1
• Profilin I
• profilin-1

Background

Profilin is an ubiquitous small (12-15 kDa) phosphoinositides and poly-L-proline binding protein that plays a role both in signal transduction pathways and actin filament dynamics. There are two mammalian profilins with similar biochemical properties but different expression patterns. Whereas profilin I appears to be highly expressed in most tissues except for skeletal muscle, profilin II is predominantly expressed in brain and at lower levels. It is also in expressed in skeletal muscle, the uterus and kidneys. Profilin is a mainly cytosolic protein with higher concentrations in dynamic membrane areas like the leading edge and ruffling membranes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PFN1