Profilin 1 Antibody (CL3524) - BSA Free

Western Blot: Profilin 1 Antibody (CL3524) [NBP2-59778] - Profilin 1 Antibody (5216) [NBP2-59778] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-PFN1 monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.

Immunohistochemistry-Paraffin: Profilin 1 Antibody (5216) [NBP2-59778] - Staining of human testis shows weak immunoreactivity in basal cells of the seminiferous ducts.

Western Blot: Profilin 1 Antibody (5216) [NBP2-59778] - Analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431 and human cell line HepG2.

Immunohistochemistry-Paraffin: Profilin 1 Antibody (5216) [NBP2-59778] - Staining of human fallopian tube shows cytoplasmic immunoreactivity in epithelial cells.

Immunohistochemistry-Paraffin: Profilin 1 Antibody (5216) [NBP2-59778] - Staining of human tonsil shows moderate to strong immunoreactivity in lymphoid cells.

Immunohistochemistry-Paraffin: Profilin 1 Antibody (5216) [NBP2-59778] - Staining of human liver shows strong immunoreactivity in circulating lymphoid cells.

Immunohistochemistry-Paraffin: Profilin 1 Antibody (5216) [NBP2-59778] - Staining of human kidney shows strong membranous combined with moderate cytoplasmic immunoreactivity in renal tubules.

Western Blot: Profilin 1 Antibody (CL3524) [NBP2-59778] - Lysates of HAP1 WT and PFN1 KO were prepared, and 50 ug of protein were processed for immunoblot with Profilin 1 antibody (NBP2-59778). The Ponceau stained transfer of the blot is shown. Antibody dilution used: NBP2-59778 at 1/1000. Predicted band size: 15 kDa. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).

• Reactivity: Hu
• Applications: WB, IHC, KD, KO
• Clone: CL3524
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Profilin 1 Antibody (CL3524) - BSA Free Summary

• Description: Novus Biologicals Knockout (KO) Validated Mouse Profilin 1 Antibody (CL3524) - BSA Free (NBP2-59778) is a monoclonal antibody validated for use in IHC and WB. Anti-Profilin 1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a recombinant protein derived from P07737, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: PFN1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Knockdown Validated
  • Knockout Validated
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  Profilin 1 Recombinant Protein Antigen (NBP2-59778PEP)

Reactivity Notes

Rat (87%), Mouse (87%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for Profilin 1 Antibody (CL3524) - BSA Free

• PFN1
• PROF1
• Profilin 1
• Profilin I
• profilin-1

Background

Profilin is an ubiquitous small (12-15 kDa) phosphoinositides and poly-L-proline binding protein that plays a role both in signal transduction pathways and actin filament dynamics. There are two mammalian profilins with similar biochemical properties but different expression patterns. Whereas profilin I appears to be highly expressed in most tissues except for skeletal muscle, profilin II is predominantly expressed in brain and at lower levels. It is also in expressed in skeletal muscle, the uterus and kidneys. Profilin is a mainly cytosolic protein with higher concentrations in dynamic membrane areas like the leading edge and ruffling membranes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Profilin 1 Antibody (NBP2-59778)(1)

Publication using NBP2-59778

• Ayoubi, R;McDowell, I;Fotouhi, M;Southern, K;McPherson, PS;Laflamme, C;NeuroSGC/YCharOS/EDDU collaborative group, ;ABIF Consortium, ; The identification of high-performing antibodies for Profilin-1 for use in Western blot, immunoprecipitation and immunofluorescence F1000Research 2023-03-29 [PMID: 37576538]

Bioinformatics

• Gene Symbol: PFN1