Profilin 1 Antibody (CL3524) - Azide and BSA Free

Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.

Staining of human liver shows strong cytoplasmic positivity in leukocytes.

Staining of human testis shows very weak cytoplasmic positivity in cells in seminiferous ducts.

Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-Profilin 1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Staining of human tonsil shows strong cytoplasmic positivity in germinal and non-germinal center cells.

Analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431 and human cell line HepG2.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: CL3524
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Profilin 1 Antibody (CL3524) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Profilin 1 Antibody (CL3524) - Azide and BSA Free (NBP2-59778) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of P07737, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: PFN1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for Profilin 1 Antibody (CL3524) - Azide and BSA Free

• PFN1
• PROF1
• Profilin 1
• Profilin I
• profilin-1

Background

Profilin is an ubiquitous small (12-15 kDa) phosphoinositides and poly-L-proline binding protein that plays a role both in signal transduction pathways and actin filament dynamics. There are two mammalian profilins with similar biochemical properties but different expression patterns. Whereas profilin I appears to be highly expressed in most tissues except for skeletal muscle, profilin II is predominantly expressed in brain and at lower levels. It is also in expressed in skeletal muscle, the uterus and kidneys. Profilin is a mainly cytosolic protein with higher concentrations in dynamic membrane areas like the leading edge and ruffling membranes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PFN1