ProDots Recombinant Human IFN-gamma Protein

ProDots® Recombinant Human IFN-gamma demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 0.15-0.75 ng/mL.

1 μg/lane of ProDots® Recombinant Human IFN-gamma was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized with silver staining, showing a single band at 17 kDa.

Product Discontinued

ProDots Recombinant Human IFN-gamma Protein Summary

• Additional Information: Supplied as ready-to-use 5 ug protein balls
• Details of Functionality: Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The ED₅₀ for this effect is 0.15-0.75 ng/mL.
• Source: E. coli-derived human IFN-gamma protein
  Gln24 - Gln166
• Accession #: CAA31639
• N-terminal Sequence: Met
• Protein/Peptide Type: ProDots Proteins
• Gene: IFNG
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 16.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 17 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt at room temperature as supplied.
  • 12 months from date of receipt at 2-8 °C as supplied.
  • 1 month at 2-8 °C under sterile conditions after reconstitution.
  • 3 months at -20 to -80 °C under sterile conditions after reconstitution.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: For a stock solution, reconstitute at 200 μg/mL in sterile deionized water, or simply roll ProDot directly into cell culture medium for immediate use.

Notes

726222.1

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ProDots Recombinant Human IFN-gamma Protein

• IFG
• IFI
• IFNG
• IFNgamma
• IFN-gamma
• Immune interferon
• interferon gamma
• interferon, gamma

Background

IFN-gamma (Interferon-gamma) is the prototype proinflammatory cytokine and is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. IFN-gamma dimers signal through a receptor complex of two IFN-gamma R1 and two IFN-gamma R2 subunits.

FAQs for IFN-gamma (PRD285). (Showing 1 - 1 of 1 FAQ).

1. Is Recombinant Human IFN-gamma Protein - Clinical grade or preclinical ?
   • We are research use only.

Bioinformatics

• Gene Symbol: IFNG
• Uniprot:
  • CAA31639()