Rat Procollagen Type 1 N-Terminal Propeptide ELISA Kit (Colorimetric)

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ELISA: Rat Procollagen Type 1 N-Terminal Propeptide ELISA Kit (Colorimetric) [NBP2-76467] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
62.50 - 4000 pg/mL
Sensitivity
37.50 pg/mL

Order Details

Rat Procollagen Type 1 N-Terminal Propeptide ELISA Kit (Colorimetric) Summary

Specificity
The Rat Procollagen Type 1 N-Terminal Propeptide ELISA Kit (Colorimetric) recognizes Rat P1NP in samples. No significant cross-reactivity or interference between Rat P1NP and analogues was observed.
Standard Curve Range
62.50 - 4000 pg/mL
Sensitivity
37.50 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.93%
Intra-Assay
CV% < 5.03%
Spike Recovery
85-102%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Certificate of Analysis
  3. Concentrated Biotinylated Detection Ab (100×)
  4. Concentrated HRP Conjugate (100×)
  5. Concentrated Wash Buffer (25×)
  6. HRP Conjugate Diluent
  7. Micro ELISA Plate(Dismountable)
  8. Plate Sealer
  9. Product Description
  10. Reference Standard & Sample Diluent
  11. Reference Standard
  12. Stop Solution
  13. Substrate Reagent

Alternate Names for Rat Procollagen Type 1 N-Terminal Propeptide ELISA Kit (Colorimetric)

  • Alpha-1 type I collagen
  • alpha1(I) procollagen
  • amino-terminal propeptide
  • CAFYD
  • COL1A1
  • collagen alpha 1 chain type I
  • collagen alpha-1(I) chain preproprotein
  • collagen alpha-1(I) chain
  • collagen of skin, tendon and bone, alpha-1 chain
  • collagen, type I, alpha 1
  • EDSARTH1
  • EDSC
  • OI1
  • OI2
  • OI3
  • OI4
  • PINP
  • pro-alpha-1 collagen type 1
  • type I proalpha 1
  • Type I Procollagen Alpha 1 Chain

Background

Procollagen type I N-terminal propeptide, also called P1NP, is a bone formation marker that is indicative of type I collagen disposition (1). P1NP is generated from the major bone protein Type I Collagen and is cleaved by proteases at the amino (N) terminal and released into the bloodstream (1-3). Typically, P1NP is released in a trimeric form, consisting of two alpha-1 chains and one alpha-2 chain, prior to being broken-down into monomers or fragments (1). Antibodies to P1NP commonly detect the trimeric structure using ELISA or radioimmunoassay applications (1). P1NP is most notably known for being a bone turnover marker, along with CTX-1, and is particularly useful for osteoporosis studies (2-4). While P1NP is used to detect bone formation, C-terminal telopeptide of type I collagen (CTX-1) is contrastingly used for bone resorption (2-4). Bone mineral density (BMD) as measured by dual-energy X-ray absorptiometry (DXA) is a common measurement of osteoporosis status and is frequently used in conjunction with P1NP and CTX-1 levels (2-4). P1NP can be collected and measured either via a blood test or urine test and is beneficial in that its levels are largely unaffected by food intake or circadian rhythms (3-4). One study of postmenopausal females found that the osteoporotic group had significantly higher average P1NP levels (51.7 ng/mL), suggestive of high bone turnover, compared to the normal range in the healthy, control group (38.9 ng/mL) and is inversely correlated with BMD (2). P1NP levels have also been shown to increase following antiresorptive therapy (3). Additionally, P1NP is considered a diagnostic marker for bone metastasis in breast cancer and prostate cancer (5). Specifically, studies found elevated P1NP serum levels is correlated with presence of bone metastasis and skeletal lesions (5).

References

1. Kuo, T. R., & Chen, C. H. (2017). Bone biomarker for the clinical assessment of osteoporosis: recent developments and future perspectives. Biomarker Research. https://doi.org/10.1186/s40364-017-0097-4

2. Kucukalic-Selimovic, E., Valjevac, A., & Hadzovic-Dzuvo, A. (2013). The utility of procollagen type 1 N-terminal propeptide for the bone status assessment in postmenopausal women. Bosnian Journal of Basic Medical Sciences. https://doi.org/10.17305/bjbms.2013.2337

3. Wheater, G., Elshahaly, M., Tuck, S. P., Datta, H. K., & van Laar, J. M. (2013). The clinical utility of bone marker measurements in osteoporosis. Journal of Translational Medicine. https://doi.org/10.1186/1479-5876-11-201

4. Williams, C., & Sapra, A. (2020). Osteoporosis Markers. In StatPearls. StatPearls Publishing.

5. D'Oronzo, S., Brown, J., & Coleman, R. (2017). The role of biomarkers in the management of bone-homing malignancies. Journal of Bone Oncology. https://doi.org/10.1016/j.jbo.2017.09.001

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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