Human Procollagen Type 1 N-Terminal Propeptide ELISA Kit (Colorimetric)

ELISA: Human Procollagen Type 1 N-Terminal Propeptide ELISA Kit (Colorimetric) [NBP2-76465] - Samples were spiked with high concentrations of Human Procollagen Type 1 N-Terminal Propeptide and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Procollagen Type 1 N-Terminal Propeptide ELISA Kit (Colorimetric) [NBP2-76465] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 15.63 - 1000 pg/mL
• Sensitivity: 9.38 pg/mL

Human Procollagen Type 1 N-Terminal Propeptide ELISA Kit (Colorimetric) Summary

• Specificity: The Human Procollagen Type 1 N-Terminal Propeptide ELISA Kit (Colorimetric) recognizes Human P1NP in samples. No significant cross-reactivity or interference between Human P1NP and analogues was observed.
• Standard Curve Range: 15.63 - 1000 pg/mL
• Sensitivity: 9.38 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.07%
• Intra-Assay: CV% < 5.05%
• Spike Recovery: 85-103%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Procollagen Type 1 N-Terminal Propeptide ELISA Kit (Colorimetric)

• Alpha-1 type I collagen
• alpha1(I) procollagen
• amino-terminal propeptide
• CAFYD
• COL1A1
• collagen alpha 1 chain type I
• collagen alpha-1(I) chain preproprotein
• collagen alpha-1(I) chain
• collagen of skin, tendon and bone, alpha-1 chain
• collagen, type I, alpha 1
• EDSARTH1
• EDSC
• OI1
• OI2
• OI3
• OI4
• PINP
• pro-alpha-1 collagen type 1
• type I proalpha 1
• Type I Procollagen Alpha 1 Chain

Background

Procollagen type I N-terminal propeptide, also called P1NP, is a bone formation marker that is indicative of type I collagen disposition (1). P1NP is generated from the major bone protein Type I Collagen and is cleaved by proteases at the amino (N) terminal and released into the bloodstream (1-3). Typically, P1NP is released in a trimeric form, consisting of two alpha-1 chains and one alpha-2 chain, prior to being broken-down into monomers or fragments (1). Antibodies to P1NP commonly detect the trimeric structure using ELISA or radioimmunoassay applications (1). P1NP is most notably known for being a bone turnover marker, along with CTX-1, and is particularly useful for osteoporosis studies (2-4). While P1NP is used to detect bone formation, C-terminal telopeptide of type I collagen (CTX-1) is contrastingly used for bone resorption (2-4). Bone mineral density (BMD) as measured by dual-energy X-ray absorptiometry (DXA) is a common measurement of osteoporosis status and is frequently used in conjunction with P1NP and CTX-1 levels (2-4). P1NP can be collected and measured either via a blood test or urine test and is beneficial in that its levels are largely unaffected by food intake or circadian rhythms (3-4). One study of postmenopausal females found that the osteoporotic group had significantly higher average P1NP levels (51.7 ng/mL), suggestive of high bone turnover, compared to the normal range in the healthy, control group (38.9 ng/mL) and is inversely correlated with BMD (2). P1NP levels have also been shown to increase following antiresorptive therapy (3). Additionally, P1NP is considered a diagnostic marker for bone metastasis in breast cancer and prostate cancer (5). Specifically, studies found elevated P1NP serum levels is correlated with presence of bone metastasis and skeletal lesions (5).

References

1. Kuo, T. R., & Chen, C. H. (2017). Bone biomarker for the clinical assessment of osteoporosis: recent developments and future perspectives. Biomarker Research. https://doi.org/10.1186/s40364-017-0097-4

2. Kucukalic-Selimovic, E., Valjevac, A., & Hadzovic-Dzuvo, A. (2013). The utility of procollagen type 1 N-terminal propeptide for the bone status assessment in postmenopausal women. Bosnian Journal of Basic Medical Sciences. https://doi.org/10.17305/bjbms.2013.2337

3. Wheater, G., Elshahaly, M., Tuck, S. P., Datta, H. K., & van Laar, J. M. (2013). The clinical utility of bone marker measurements in osteoporosis. Journal of Translational Medicine. https://doi.org/10.1186/1479-5876-11-201

4. Williams, C., & Sapra, A. (2020). Osteoporosis Markers. In StatPearls. StatPearls Publishing.

5. D'Oronzo, S., Brown, J., & Coleman, R. (2017). The role of biomarkers in the management of bone-homing malignancies. Journal of Bone Oncology. https://doi.org/10.1016/j.jbo.2017.09.001

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Procollagen Type 1 N-Terminal Propeptide ELISA Kit (NBP2-76465)(2)

Publications using NBP2-76465

• Kristen J. Koltun, Adam J. Sterczala, Nicole M. Sekel, Kellen T. Krajewski, Brian J. Martin, Mita Lovalekar, Christopher Connaboy, Shawn D. Flanagan, Sophie L. Wardle, Thomas J. O'Leary, Julie P. Greeves, Bradley C. Nindl Effect of acute resistance exercise on bone turnover in young adults before and after concurrent resistance and interval training Physiological Reports 2024-01-31 [PMID: 38296351]
• Zheng SW, Sun CH, Wen ZJ et al. Decreased serum CXCL12/SDF-1 concentrations may reflect disease severity of non-traumatic osteonecrosis of femoral head Clinica chimica acta; international journal of clinical chemistry 2022-02-17 [PMID: 35183527]