proBDNF Antibody - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: proBDNF Antibody [NB100-98754] - IF on rat hypophysis at 40 ug/ml concentration using Sheep antibody to rat, mouse, zebra fish proBDNF (pro BDNF, pro Brain-derived neurotrophic factor): IgG, DAPI counterstained.

Immunocytochemistry/Immunofluorescence: proBDNF Antibody [NB100-98754] - Rat hypophysis DAPI stained.

• Reactivity: Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

proBDNF Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Sheep proBDNF Antibody - Azide and BSA Free (NB100-98754) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-proBDNF Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide from rat proBDNF conjugated to blue carrier protein was used as the antigen. The peptide is homologous in mouse and zebrafish and shares 96% identity with human sequence.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: BDNF
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 10-50 ug/ml
  • Immunohistochemistry-Paraffin 10-50 ug/ml
  • Western Blot 10-50 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Ammonium sulfate precipitation
• Reconstitution Instructions: Reconstitute 0.5 mg size in 0.5 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the 0.1 mg size is provided in reconstituted format.

Alternate Names for proBDNF Antibody - Azide and BSA Free

• abrineurin
• brain-derived neurotrophic factor
• MGC34632
• neurotrophin

Background

FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for proBDNF Antibody (NB100-98754)(1)

Publication using NB100-98754

• Mohammadi M, Manaheji H, Maghsoudi N et al. Microglia dependent BDNF and proBDNF can impair spatial memory performance during persistent inflammatory pain Behav. Brain Res. 2020-05-19 [PMID: 32442548] (WB, Rat)

Bioinformatics

• Gene Symbol: BDNF
• Entrez:
  • 12064(Mouse)
  • 24225(Rat)
• Uniprot:
  • P23560()