PRKCDBP Antibody - Azide and BSA Free

Immunohistochemistry: PRKCDBP Antibody [NBP1-44091] - Tissue: Placenta. Fixation: formalin fixed paraffin embedded. Primary antibody: Anti-Protein Kinase C delta Binding Protein at 5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: Protein Kinase C delta Binding Protein as precipitated red signal with hematoxylin purple nuclear counterstain.

Immunohistochemistry: PRKCDBP Antibody [NBP1-44091] - Tissue: Lung. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Incubated at 5 ug/mL for 1 h at RT.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PRKCDBP Antibody - Azide and BSA Free Summary

• Description: This antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: PRKCDBP antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminal region of human PRKCDBP. (Uniprot: Q969G5)
• Specificity: A BLAST analysis was used to suggest cross-reactivity with rat, human, and mouse based on 100% sequence homology. Cross-reactivity with PRKCDBP from other sources has not been determined.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CAVIN3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunohistochemistry 5 ug/ml
  • Western Blot 1ug/ml
• Application Notes: This product is tested in immunohistochemistry and ELISA and useful for Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~37kDa corresponding to the appropriate cell lysate or extract.

Reactivity Notes

A BLAST analysis was used to suggest cross-reactivity with rat, human, and mouse based on 100% sequence homology. Cross-reactivity with PRKCDBP from other sources has not been determined.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 30% Glycerol
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Alternate Names for PRKCDBP Antibody - Azide and BSA Free

• Cavin-3
• HSRBC
• MGC20400
• protein kinase C, delta binding protein
• sdr-related gene product that binds to c-kinase
• Serum deprivation response factor-related gene product that binds to C-kinase
• SRBCprotein kinase C delta-binding protein

Background

The protein encoded by this gene was identified as a binding protein of the protein kinase C, delta (PRKCD). The expression of this gene in cultured cell lines is strongly induced by serum starvation. The expression of this protein was found to be down-regulated in various cancer cell lines, suggesting the possible tumor suppressor function of this protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PRKCDBP Antibody (NBP1-44091)(4)

Publications using NBP1-44091

• McMahon KA, Stroud DA, Gambin Y et al. Cavin3 released from caveolae interacts with BRCA1 to regulate the cellular stress response eLife 2021-06-18 [PMID: 34142659]
• Bastiani M, Liu L, Hill MM et al. MURC/Cavin-4 and cavin family members form tissue-specific caveolar complexes. J Cell Biol 2009-06-01 [PMID: 19546242]
• McMahon KA, Zajicek H, Li WP et al. SRBC/cavin-3 is a caveolin adapter protein that regulates caveolae function. EMBO J 2009-04-22 [PMID: 19262564]
• Izumi Y, Hirai S, Tamai Y, Fujise-Matsuoka A, Nishimura Y. Ohno S A protein kinase Cdelta-binding protein SRBC whose expression is induced by serum starvation. J Biol Chem;272(11):7381-9. 1997-03-14 [PMID: 9054438]

Bioinformatics

• Gene Symbol: CAVIN3
• Entrez:
  • 112464(Human)
• Uniprot:
  • Q969G5(Human)