Presenilin-1 Antibody - BSA Free

Western Blot: Presenilin-1 Antibody [NBP1-76792] - Human brain lysate with presenilin1 antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.

Immunocytochemistry/Immunofluorescence: Presenilin-1 Antibody [NBP1-76792] - Cell surface staining of VDR (green) on live neurons followed by fixation and immunofluorescence labeling of target protein (100x) Presenilin-1. Overlay images indicate the possible co-localization of limited co-localization of VDR/ Presenilin-1 on the neuronal plasma membrane. Image collected and cropped by CiteAb from the following publication (//journals.plos.org/plosone/article?id=10.1371/journal.pone.0188605) licensed under a CC-BY license.

Immunocytochemistry/ Immunofluorescence: Presenilin-1 Antibody - BSA Free [NBP1-76792] - Immunofluorescence of Presenilin-1 in Human Brain cells with Presenilin-1 antibody at 20 ug/mL.

Immunohistochemistry: Presenilin-1 Antibody - BSA Free [NBP1-76792] - Immunohistochemistry of Presenilin-1 in human brain tissue with Presenilin-1 antibody at 2.5 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Presenilin-1 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 23 amino acid synthetic peptide from near the carboxy terminus of human presenilin1. The immunogen is located within amino acids 330 - 380 of Presenilin1. Amino Acid Squence: SEEWEAQRDSHLGPHRSTPESRA
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PSEN1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 0.5-2 ug/ml
• Control:

  Human Brain Cerebral Cortex Membrane Tissue Lysate (Adult Membrane Normal)

• Control Peptide:

  Presenilin-1 Peptide (NBP1-76792PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (86%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for Presenilin-1 Antibody - BSA Free

• AD3EC 3.4.23.-
• Alzheimer disease 3
• EC 3.4.23
• FAD
• presenilin 1
• Presenilin1
• Presenilin-1
• Protein S182
• PS-1
• PS1presenilin-1
• PSEN1
• PSNL1
• S182

Background

Presenilin1 was initially identified a marker of susceptibility to early-onset Alzheimer's disease. In addition to PEN2, nicastrin and APH-1, Presenilin1 forms the gamma-secretase protein complex, a membrane-bound aspartyl protease that can cleave certain proteins at peptide bonds buried within the hydrophobic environment of the lipid bilayer. This cleavage is responsible for a key step in signaling from several cell-surface receptors and is thought to be required for the generation of the neurotoxic amyloid peptides that are central to the pathogenesis of Alzheimer's disease. Like the tumor necrosis factor-alpha-converting enzyme (TACE) and the beta-site cleavage enzyme (BACE) protease families, gamma-secretase will cleave the amyloid precursor protein (APP), but within the intramembrane region of APP, resulting in either the non-toxic p3 (from the alpha and gamma cleavage site) or the toxic Abeta amyloid peptide (from the beta and gamma cleavage site). It is thought that accumulation of the Abeta peptide is the precursor to Alzheimer's disease. Multiple isoforms of presenilin1 are known to exist. This antibody has no cross-reactivity to presenilin2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Presenilin-1 Antibody (NBP1-76792)(1)

Publication using NBP1-76792

• Dursun E. , Gezen-Ak D. Vitamin D receptor is present on the neuronal plasma membrane and is co-localized with amyloid precursor protein, ADAM10 or Nicastrin. PLoS One 2017-11-28 [PMID: 29176823] (ICC/IF)

Bioinformatics

• Gene Symbol: PSEN1
• Uniprot:
  • NP_000012(Human)