APH1A Antibody

Western Blot: APH1A Antibody [NB100-74360] - Analysis of mouse brain and kidney extracts.

Immunocytochemistry/Immunofluorescence: APH1A Antibody [NB100-74360] - Staining of APH-1 in human KNS-42 glioma cells.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC, IP, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.69 mg/ml

APH1A Antibody Summary

• Immunogen: Synthetic peptide, KLH conjugated, corresponding to residues C (245) R R Q E D S R V M V Y S A L R I P P E D (265) of human APH-1aL/CT.
• Specificity: APH-1
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: APH1A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) 1:10-1:500
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:1000
• Application Notes: WB: Detects an approx. 18 kDa protein representing APH-1 in mouse brain and kidney extracts. ChIP usage was reported in the scientific literature (PMID: 20145246).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.005% Sodium Azide
• Concentration: 0.69 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for APH1A Antibody

• 6530402N02Rik
• anterior pharynx defective 1 homolog A (C. elegans)
• APH-1
• APH1A
• APH-1A
• aph-1alpha
• CGI-78
• gamma-secretase subunit APH-1A
• Presenilin-stabilization factor
• PSF

Background

Anterior Pharynx Defective 1 (APH1a) is an integral transmembrane protein forming a component of the gamma-secretase complex, a complex composed of a presenilin homodimer (PSEN1 or PSEN2), nicastrin (NCSTN), APH1 (APH1A or APH1B) and PEN2. The gamma-secretase complex is an endoprotease complex that catalyzes the intramembrane cleavage of integral proteins such as Notch receptors and APP (beta-amyloid precursor protein).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for APH1A Antibody (NB100-74360)(2)

Publications using NB100-74360

• Area-Gomez E, de Groof AJ et al. Presenilins are enriched in endoplasmic reticulum membranes associated with mitochondria. Am J Pathol;175(5):1810-6. 2009-11-01 [PMID: 19834068]
• Gu Y, Chen F et al. APH-1 interacts with mature and immature forms of presenilins and nicastrin and may play a role in maturation of presenilin.nicastrin complexes. J Biol Chem;278(9):7374-80. 2003-02-28 [PMID: 12471034]

Bioinformatics

• Gene Symbol: APH1A
• Entrez:
  • 51107(Human)
  • 235606(Mouse)
• Uniprot:
  • Q96BI3()