Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse PRDM16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6295) ...read more
PRDM16 was detected in immersion fixed frozen sections of mouse embryo (E13.5) using Sheep Anti-Human/Mouse PRDM16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6295) at 10 µg/mL overnight at 4 °C. Tissue ...read more
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Sterile PBS to a final concentration of 0.2 mg/mL.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for PRDM16/MEL1 Antibody [Unconjugated]
MDS1/EVI1-like gene 1
PR domain containing 16
PR domain zinc finger protein 16
PR domain-containing protein 16
Transcription factor MEL1
PRDM16 (PR [PRDI-BF1 and RIZ] domain containing protein 16; also MEL-1) is a 170 kDa member of the PR Domain family of proteins. It is a transcriptional regulator expressed in the embryo, and is reported to participate in the maintenance of both neuronal and hematopoietic progenitor stem cells populations, and to preferentially promote the development of brown fat from adipomyocyte precursors. The generation of brown fat is likely due to suppression of muscle-specific factors. Mouse PRDM16 is 1275 amino acids (aa) in length. It contains one SET domain (aa 85-208) followed by ten C2H2 type Zn finger motifs (aa 230-1030). There are multiple potential isoform variants that likely vary from 150-170 kDa in size. One isoform shows a deletion of aa 1232-1250, a second isoform shows a three aa substitution for aa 1174-1275, and a third isoform possesses an alternative start site at Met21, coupled to a deletion of aa 1196-1133. Over aa 537-688, mouse PRDM16 shares 81% and 95% aa identity with human and rat PRDM16, respectively.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.