PRDM16/MEL1 Antibody [Unconjugated]

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse PRDM16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6295) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PRDM16 at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

PRDM16 was detected in immersion fixed frozen sections of mouse embryo (E13.5) using Sheep Anti-Human/Mouse PRDM16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6295) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (orange, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to the trigeminal ganglion. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

PRDM16/MEL1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse PRDM16
  Lys537-Glu688
  Accession # A2A935.1
• Specificity: Detects mouse and human PRDM16 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: PRDM16
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • Immunohistochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PRDM16/MEL1 Antibody [Unconjugated]

• MDS1/EVI1-like gene 1
• MEL1
• MEL1KIAA1675PFM13MGC166915
• PFM13
• PR domain containing 16
• PR domain zinc finger protein 16
• PR domain-containing protein 16
• PRDM16
• Transcription factor MEL1

Background

PRDM16 (PR [PRDI-BF1 and RIZ] domain containing protein 16; also MEL-1) is a 170 kDa member of the PR Domain family of proteins. It is a transcriptional regulator expressed in the embryo, and is reported to participate in the maintenance of both neuronal and hematopoietic progenitor stem cells populations, and to preferentially promote the development of brown fat from adipomyocyte precursors. The generation of brown fat is likely due to suppression of muscle-specific factors. Mouse PRDM16 is 1275 amino acids (aa) in length. It contains one SET domain (aa 85-208) followed by ten C2H2 type Zn finger motifs (aa 230-1030). There are multiple potential isoform variants that likely vary from 150-170 kDa in size. One isoform shows a deletion of aa 1232-1250, a second isoform shows a three aa substitution for aa 1174-1275, and a third isoform possesses an alternative start site at Met21, coupled to a deletion of aa 1196-1133. Over aa 537-688, mouse PRDM16 shares 81% and 95% aa identity with human and rat PRDM16, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PRDM16/MEL1 Antibody (AF6295)(11)

Publications using AF6295

• WK Lau, NAA Noruddin, AH Ariffin, MZ Mahmud, MHM Noor, A Amanah, MF Hamzah, Z Zafarina Novel discovery of Averrhoa bilimbi ethanolic leaf extract in the stimulation of brown fat differentiation program in combating diet-induced obesity BMC Complement Altern Med, 2019;19(1):243. 2019 [PMID: 31488120] (Screening, Human)
• Q Chen, L Huang, D Pan, LJ Zhu, YX Wang Cbx4 Sumoylates Prdm16 to Regulate Adipose Tissue Thermogenesis Cell Rep, 2018;22(11):2860-2872. 2018 [PMID: 29539416] (Western Blot, Mouse)
• S Groman-Lup, J Adewumi, KU Park, JA Brzezinski The Transcription Factor Prdm16 Marks a Single Retinal Ganglion Cell Subtype in the Mouse Retina Invest. Ophthalmol. Vis. Sci., 2017;58(12):5421-5433. 2017 [PMID: 29053761] (IHC-Fr, Mouse)
• D Bauters, M Cobbaut, L Geys, J Van Lint, B Hemmeryckx, HR Lijnen Loss of ADAMTS5 enhances brown adipose tissue mass and promotes browning of white adipose tissue via CREB signaling Mol Metab, 2017;6(7):715-724. 2017 [PMID: 28702327] (Western Blot, Mouse)
• L Huang, D Pan, Q Chen, LJ Zhu, J Ou, M Wabitsch, YX Wang Transcription factor Hlx controls a systematic switch from white to brown fat through Prdm16-mediated co-activation Nat Commun, 2017;8(1):68. 2017 [PMID: 28701693] (Immunoprecipitation, Mouse)
• Hanying Ding Fasting induces a subcutaneous-to-visceral fat switch mediated by microRNA-149-3p and suppression of PRDM16 Nat Commun, 2016;7(0):11533. 2016 [PMID: 27240637] (Western Blot, Mouse)
• Moisan A, Lee Y, Zhang J, Hudak C, Meyer C, Prummer M, Zoffmann S, Truong H, Ebeling M, Kiialainen A, Gerard R, Xia F, Schinzel R, Amrein K, Cowan C White-to-brown metabolic conversion of human adipocytes by JAK inhibition. Nat Cell Biol, 2015;17(1):57-67. 2015 [PMID: 25487280] (Western Blot, Human)
• Pan, Dongning, Mao, Chunxiao, Quattrochi, Brian, Friedline, Randall, Zhu, Lihua J, Jung, Dae Youn, Kim, Jason K, Lewis, Brian, Wang, Yong-Xu MicroRNA-378 controls classical brown fat expansion to counteract obesity. Nat Commun, 2014;5(0):4725. 2014 [PMID: 25145289] (Western Blot, Mouse)
• Chou C, Lin Y, Wang H, Zhu X, Giovarelli M, Briata P, Gherzi R, Garvey W, Chen C KSRP ablation enhances brown fat gene program in white adipose tissue through reduced miR-150 expression. Diabetes, 2014;63(9):2949-61. 2014 [PMID: 24722250] (Western Blot, Mouse)
• Trajkovski, Mirko, Ahmed, Kashan, Esau, Christin, Stoffel, Markus MyomiR-133 regulates brown fat differentiation through Prdm16. Nat Cell Biol, 2012;14(12):1330-5. 2012 [PMID: 23143398] (Western Blot, Mouse)
• Qiang L, Wang L, Kon N, Zhao W, Lee S, Zhang Y, Rosenbaum M, Zhao Y, Gu W, Farmer S, Accili D Brown remodeling of white adipose tissue by SirT1-dependent deacetylation of Ppargamma. Cell, 2012;150(3):620-32. 2012 [PMID: 22863012] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: PRDM16
• Uniprot:
  • A2A935.1()