PRDM16/MEL1 Antibody [Unconjugated]

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Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse PRDM16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6295) ...read more
PRDM16 was detected in immersion fixed frozen sections of mouse embryo (E13.5) using Sheep Anti-Human/Mouse PRDM16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6295) at 10 µg/mL overnight at 4 °C. Tissue ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

PRDM16/MEL1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse PRDM16
Lys537-Glu688
Accession # A2A935.1
Specificity
Detects mouse and human PRDM16 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
PRDM16
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Publications
Read Publications using
AF6295 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PRDM16/MEL1 Antibody [Unconjugated]

  • MDS1/EVI1-like gene 1
  • MEL1
  • MEL1KIAA1675PFM13MGC166915
  • PFM13
  • PR domain containing 16
  • PR domain zinc finger protein 16
  • PR domain-containing protein 16
  • PRDM16
  • Transcription factor MEL1

Background

PRDM16 (PR [PRDI-BF1 and RIZ] domain containing protein 16; also MEL-1) is a 170 kDa member of the PR Domain family of proteins. It is a transcriptional regulator expressed in the embryo, and is reported to participate in the maintenance of both neuronal and hematopoietic progenitor stem cells populations, and to preferentially promote the development of brown fat from adipomyocyte precursors. The generation of brown fat is likely due to suppression of muscle-specific factors. Mouse PRDM16 is 1275 amino acids (aa) in length. It contains one SET domain (aa 85-208) followed by ten C2H2 type Zn finger motifs (aa 230-1030). There are multiple potential isoform variants that likely vary from 150-170 kDa in size. One isoform shows a deletion of aa 1232-1250, a second isoform shows a three aa substitution for aa 1174-1275, and a third isoform possesses an alternative start site at Met21, coupled to a deletion of aa 1196-1133. Over aa 537-688, mouse PRDM16 shares 81% and 95% aa identity with human and rat PRDM16, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PRDM16/MEL1 Antibody (AF6295)(10)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: IHC-Fr, Immunoprecipitation, Western Blot.


Filter By Application
IHC-Fr
(1)
Immunoprecipitation
(1)
Western Blot
(8)
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Filter By Species
Human
(1)
Mouse
(9)
All Species
Showing Publications 1 - 10 of 10.
Publications using AF6295 Applications Species
Q Chen, L Huang, D Pan, LJ Zhu, YX Wang Cbx4 Sumoylates Prdm16 to Regulate Adipose Tissue Thermogenesis Cell Rep, 2018;22(11):2860-2872. 2018 [PMID: 29539416] (Western Blot, Mouse) Western Blot Mouse
S Groman-Lup, J Adewumi, KU Park, JA Brzezinski The Transcription Factor Prdm16 Marks a Single Retinal Ganglion Cell Subtype in the Mouse Retina Invest. Ophthalmol. Vis. Sci., 2017;58(12):5421-5433. 2017 [PMID: 29053761] (IHC-Fr, Mouse) IHC-Fr Mouse
D Bauters, M Cobbaut, L Geys, J Van Lint, B Hemmeryckx, HR Lijnen Loss of ADAMTS5 enhances brown adipose tissue mass and promotes browning of white adipose tissue via CREB signaling Mol Metab, 2017;6(7):715-724. 2017 [PMID: 28702327] (Western Blot, Mouse) Western Blot Mouse
L Huang, D Pan, Q Chen, LJ Zhu, J Ou, M Wabitsch, YX Wang Transcription factor Hlx controls a systematic switch from white to brown fat through Prdm16-mediated co-activation Nat Commun, 2017;8(1):68. 2017 [PMID: 28701693] (Immunoprecipitation, Mouse) Immunoprecipitation Mouse
Hanying Ding Fasting induces a subcutaneous-to-visceral fat switch mediated by microRNA-149-3p and suppression of PRDM16 Nat Commun, 2016;7(0):11533. 2016 [PMID: 27240637] (Western Blot, Mouse) Western Blot Mouse
Moisan A, Lee Y, Zhang J, Hudak C, Meyer C, Prummer M, Zoffmann S, Truong H, Ebeling M, Kiialainen A, Gerard R, Xia F, Schinzel R, Amrein K, Cowan C White-to-brown metabolic conversion of human adipocytes by JAK inhibition. Nat Cell Biol, 2015;17(1):57-67. 2015 [PMID: 25487280] (Western Blot, Human) Western Blot Human
Pan, Dongning, Mao, Chunxiao, Quattrochi, Brian, Friedline, Randall, Zhu, Lihua J, Jung, Dae Youn, Kim, Jason K, Lewis, Brian, Wang, Yong-Xu MicroRNA-378 controls classical brown fat expansion to counteract obesity. Nat Commun, 2014;5(0):4725. 2014 [PMID: 25145289] (Western Blot, Mouse) Western Blot Mouse
Chou C, Lin Y, Wang H, Zhu X, Giovarelli M, Briata P, Gherzi R, Garvey W, Chen C KSRP ablation enhances brown fat gene program in white adipose tissue through reduced miR-150 expression. Diabetes, 2014;63(9):2949-61. 2014 [PMID: 24722250] (Western Blot, Mouse) Western Blot Mouse
Trajkovski, Mirko, Ahmed, Kashan, Esau, Christin, Stoffel, Markus MyomiR-133 regulates brown fat differentiation through Prdm16. Nat Cell Biol, 2012;14(12):1330-5. 2012 [PMID: 23143398] (Western Blot, Mouse) Western Blot Mouse
Qiang L, Wang L, Kon N, Zhao W, Lee S, Zhang Y, Rosenbaum M, Zhao Y, Gu W, Farmer S, Accili D Brown remodeling of white adipose tissue by SirT1-dependent deacetylation of Ppargamma. Cell, 2012;150(3):620-32. 2012 [PMID: 22863012] (Western Blot, Mouse) Western Blot Mouse

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Bioinformatics

Gene Symbol PRDM16
Uniprot