Western Blot: CEBP Beta Antibody (47A1) [NBP1-04266] - NIH 3T3 cell lysates were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CEBP Beta; antibody (1:1,000). Protein was visualized using ...read more
Western Blot: CEBP Beta Antibody (47A1) [NBP1-04266] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated by 4-12% SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% ...read more
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS (pH 7.4) and 10% Glycerol
0.02% Sodium Azide
Protein G purified
Alternate Names for CEBP Beta Antibody (47A1)
CCAAT/enhancer binding protein (C/EBP), beta
CCAAT/enhancer-binding protein beta
interleukin 6-dependent DNA-binding protein
Liver activator protein
liver-enriched transcriptional activator protein
Nuclear factor NF-IL6
nuclear factor of interleukin 6
Transcription factor 5
CCAAT/enhancer-binding proteins (C/EBPs) are a family of basic leucine zipper (bZIP) DNA binding proteins that play important roles in normal cellular differentiation and function in a variety of tissues. The C/EBP family is critical for innate immunity and inflammatory response, and consists of five core members: C/EBP alpha, C/EBP beta, C/EBP delta, C/EBP epsilon, and C/EBP gamma. Among these proteins, C/EBP beta is usually highly expressed in liver, intestine, lung and adipose. C/EBP beta has three isoforms, two activator, (LAP* and LAP) and a repressor (LIP). These isoforms are differentially translated from the same mRNA. The expression of these isoforms is developmentally and hormonally regulated.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
3T3-L1 mouse embryonic fibroblast adipose-like cell line
Western Blot: CEBP beta Antibody (47A1) [NBP1-04266] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated on a 4-12% gel by SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. The membrane was probed with anti-CEBP beta 1:1000 in non-fat milk.
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