CEBP Beta Antibody (47A1)

Images

 
Western Blot: CEBP Beta Antibody (47A1) [NBP1-04266] - NIH 3T3 cell lysates were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CEBP Beta; antibody (1:1,000). Protein was visualized using ...read more
Western Blot: CEBP Beta Antibody (47A1) [NBP1-04266] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated by 4-12% SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA
Clone
47A1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

CEBP Beta Antibody (47A1) Summary

Immunogen
Recombinant human C/EBP-beta (1-271 aa) purified from E. coli
Specificity
Anti-human C/EBP beta mAb, clone 47A1, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human C/EBP beta protein.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CEBPB
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:100-1:2000
Reviewed Applications
Read 1 Review rated 5
using
NBP1-04266 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for CEBP Beta Antibody (47A1)

  • C/EBP beta
  • C/EBP-beta
  • CCAAT/enhancer binding protein (C/EBP), beta
  • CCAAT/enhancer-binding protein beta
  • CRP2
  • IL6DBP
  • interleukin 6-dependent DNA-binding protein
  • LAPTCF-5
  • Liver activator protein
  • liver-enriched transcriptional activator protein
  • MGC32080
  • NF-IL6
  • Nuclear factor NF-IL6
  • nuclear factor of interleukin 6
  • TCF5NFIL6
  • Transcription factor 5

Background

CCAAT/enhancer-binding proteins (C/EBPs) are a family of basic leucine zipper (bZIP) DNA binding proteins that play important roles in normal cellular differentiation and function in a variety of tissues. The C/EBP family is critical for innate immunity and inflammatory response, and consists of five core members: C/EBP alpha, C/EBP beta, C/EBP delta, C/EBP epsilon, and C/EBP gamma. Among these proteins, C/EBP beta is usually highly expressed in liver, intestine, lung and adipose. C/EBP beta has three isoforms, two activator, (LAP* and LAP) and a repressor (LIP). These isoforms are differentially translated from the same mRNA. The expression of these isoforms is developmentally and hormonally regulated.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CEBP Beta Antibody (NBP1-04266) (0)

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Review for CEBP Beta Antibody (NBP1-04266) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP1-04266:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot CEBP Beta NBP1-04266
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reviewed by:
Anonymous
WB Mouse 03/27/2018
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Summary

ApplicationWestern Blot
Sample Tested3T3-L1 mouse embryonic fibroblast adipose-like cell line
SpeciesMouse
LotA083501

Comments

CommentsWestern Blot: CEBP beta Antibody (47A1) [NBP1-04266] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated on a 4-12% gel by SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. The membrane was probed with anti-CEBP beta 1:1000 in non-fat milk.

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FAQs for CEBP Beta Antibody (NBP1-04266) (0)

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Secondary Antibodies

 

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Diseases for CEBP Beta Antibody (NBP1-04266)

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Blogs on CEBP Beta.

Transcription Factor Antibodies Used In Landmark Evolutionary Study
We at Novus Biologicals offer a full antibody database targeted to transcription factor research. Recently, CEBP antibodieswere used in a research study exploring the evolution of gene regulation in various vertebrates. The results revealed surprising...  Read full blog post.

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Recent Reviews

5
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1
4
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2
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1
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Anonymous
03/27/2018
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol CEBPB
Uniprot