Podoplanin Antibody (18H5)

Images

 
Western Blot: Podoplanin Antibody (18H5) [NB600-1013] - Fig. 1: Western blot analysis of Podoplanin expression in human lymphatic endothelial cells (LEC) and HUVECs. Total lysate of both cell types were subjected to ...read more
Immunocytochemistry/ Immunofluorescence: Podoplanin Antibody (18H5) [NB600-1013] - staining (green) of Podoplanin in primary human dermal lymphatic endothelial cells (HDLEC) with anti-human Podoplanin (0,5ug/ml) and ...read more
Flow Cytometry: Podoplanin Antibody (18H5) [NB600-1013] - analysis with human primary lymphatic endothelial cells (HDLEC). As secondary antibody anti-mouse IgG-PE was used.
Flow Cytometry: Podoplanin Antibody (18H5) [NB600-1013] - Fig. 1: Flow analysis of Podoplanin expression in primary human dermal mivrovascular endothelial cells (HDMVEC) and human umbilical vein endothelial cells ...read more
Flow Cytometry: Podoplanin Antibody (18H5) [NB600-1013] - Analysis using Biotin conjugate of NB600-1013. Analysis with primary human dermal microvascular endothelial cells (HDMVEC). Upper panel: no primary antibody; ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready
Clone
18H5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Podoplanin Antibody (18H5) Summary

Immunogen
MDCK cells stably expressing gp36 (Podoplanin) protein
Localization
Cell Membrane
Marker
Lymphatic Endothelium Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PDPN
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-5 ug/ml
  • Flow Cytometry 5-10 ug/ml
  • Immunocytochemistry/Immunofluorescence 6-30 ug/ml
  • Immunohistochemistry 6 - 30 ug/ml
  • CyTOF-ready
Application Notes
This antibody is Cytof ready.
Control
Podoplanin Lysate (NBP2-65884)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein G purified
Reconstitution Instructions
Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of > 0.5 mg/ml

Alternate Names for Podoplanin Antibody (18H5)

  • 36-KD
  • Aggrus
  • Gp36
  • Gp38
  • GP40
  • HT1A-1
  • hT1alpha-1
  • hT1alpha-2
  • lung type I cell membrane associated glycoprotein
  • lung type-I cell membrane-associated glycoprotein (T1A-2)
  • OTS 8
  • OTS8
  • PA2.26 antigen
  • PDPN
  • Podoplanin
  • RANDAM-2
  • T1A
  • T1A-2
  • T1-alpha

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Podoplanin Antibody (NB600-1013) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB600-1013AF405
Alexa Fluor 488 Labeled NB600-1013AF488
Alexa Fluor 647 Labeled NB600-1013AF647
Alexa Fluor 700 Labeled NB600-1013AF700
Allophycocyanin Labeled NB600-1013APC
Biotin Labeled NBP1-18600
DyLight 350 Labeled NB600-1013UV
DyLight 405 Labeled NB600-1013V
DyLight 488 Labeled NB600-1013G
DyLight 680 Labeled NB600-1013FR
DyLight 755 Labeled NB600-1013IR
PE Labeled NB600-1013PE
PerCP Labeled NB600-1013PCP

Additional Array Products

Bioinformatics Tool for Podoplanin Antibody (NB600-1013)

Discover related pathways, diseases and genes to Podoplanin Antibody (NB600-1013). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Podoplanin Antibody (NB600-1013)

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Pathways for Podoplanin Antibody (NB600-1013)

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PTMs for Podoplanin Antibody (NB600-1013)

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Research Areas for Podoplanin Antibody (NB600-1013)

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Blogs on Podoplanin.

Meningeal lymphatics: recent discovery defying the concept of central nervous system 'immune privilege'
By Jennifer Sokolowski, MD, PhD. Identification and characterization of meningeal lymphaticsThe recent discovery of a lymphatic system in the meninges surrounding the brain and spinal cord has spurred a surge of int...  Read full blog post.

Podoplanin (OST8, Glycoprotein (Gp) 36 or 38, Lung Type I Cell Membrane Associated Glycoprotein)
Podoplanin is a mucin-type 1 transmembrane glycoprotein found in a wide range of tissues. It appears to be differentially expressed in endothelial cells of lymphatic but not blood vessel origin. In normal skin and kidney, podoplanin co-localizes with...  Read full blog post.

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Bioinformatics

Gene Symbol PDPN