Podocalyxin Like Antibody [Biotin]

• Reactivity: Mu
• Applications: WB, Flow, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Podocalyxin Like Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Podocalyxin
  His22-Arg402
  Accession # Q9R0M4
• Specificity: Detects mouse Podocalyxin in Western blots. In Western blots, less than 2% cross-reactivity with recombinant human (rh) Podocalyxin and rhEndoglycan is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PODXL
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Podocalyxin Like Antibody [Biotin]

• GCTM
• Gp200MGC138240
• PCLP
• PCLP1
• PCLP-1
• PCLPGCTM-2 antigen
• PCpodocalyxin
• POD XL
• Podocalyxin
• Podocalyxin-like protein 1
• podocalyxin-like
• PODXL

Background

Podocalyxin, also known as Podocalyxin-like protein-1 (PCLP1 or PODXL), is a type I transmembrane glycoprotein. It belongs to the CD34/Podocalyxin family of sialomucins that share structural similarity and sequence homology. Podocalyxin is a major sialoprotein in the podocytes of the kidney glomerulus and is also expressed by both endothelium and multipotent hematopoietic progenitors. It has been identified as a novel cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells (1, 2).

1. Li, J. et al. (2001) DNA Seq. 12(5):407.
2. Hara, T. et al. (1999) Immunity 11(5):567.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Podocalyxin Like Antibody (BAF1556)(8)

Publications using BAF1556

• Mise, K;Long, J;Galvan, DL;Ye, Z;Fan, G;Sharma, R;Serysheva, II;Moore, TI;Jeter, CR;Anna Zal, M;Araki, M;Wada, J;Schumacker, PT;Chang, BH;Danesh, FR; NDUFS4 regulates cristae remodeling in diabetic kidney disease Nature communications 2024-03-04 [PMID: 38438382] (MACS, Mouse)
• Li, L;Long, J;Mise, K;Poungavrin, N;Lorenzi, PL;Mahmud, I;Tan, L;Saha, PK;Kanwar, YS;Chang, BH;Danesh, FR; The Transcription Factor ChREBP Links Mitochondrial Lipidomes to Mitochondrial Morphology and Progression of Diabetic Kidney Disease The Journal of biological chemistry 2023-08-21 [PMID: 37611830] (Cell Selection, Mouse)
• Danesh, F;Mise, K;Long, J;Galvan, D;Ye, Z;Fan, G;Serysheva, I;Moore, T;Wada, J;Schumacker, P;Chang, B; NDUFS4 Regulates Cristae Remodeling in Diabetic Kidney Disease Research square 2023-06-30 [PMID: 37461606] (Cell Isolation, Mouse)
• L Li, J Long, K Mise, DL Galvan, PA Overbeek, L Tan, SV Kumar, WK Chan, PL Lorenzi, BH Chang, FR Danesh PGC1alpha is required for the renoprotective effect of lncRNA Tug1 in�vivo and links Tug1 with urea cycle metabolites Cell Reports, 2021-08-10;36(6):109510. 2021-08-10 [PMID: 34380028] (Flow Cytometry, Mouse)
• J Long, DL Galvan, K Mise, YS Kanwar, L Li, N Poungvarin, PA Overbeek, BH Chang, FR Danesh Role for carbohydrate response element-binding protein (ChREBP) in high glucose-mediated repression of long noncoding RNA Tug1 J. Biol. Chem., 2020-05-28;0(0):. 2020-05-28 [PMID: 32467232] (Cell Isolation, Mouse)
• Hélène Lazareth, Carole Henique, Olivia Lenoir, Victor G. Puelles, Martin Flamant, Guillaume Bollée et al. The tetraspanin CD9 controls migration and proliferation of parietal epithelial cells and glomerular disease progression Nature Communications 2019-07-24 [PMID: 31341160]
• Sathyanarayana P, Houde E, Marshall D, Volk A, Makropoulos D, Emerson C, Pradeep A, Bugelski PJ, Wojchowski DM CNTO 530 functions as a potent EPO mimetic via unique sustained effects on bone marrow proerythroblast pools. Blood, 2009-03-05;113(20):4955-62. 2009-03-05 [PMID: 19264917] (Flow Cytometry, Mouse)
• Sathyanarayana P, Menon MP, Bogacheva O, Bogachev O, Niss K, Kapelle WS, Houde E, Fang J, Wojchowski DM Erythropoietin modulation of podocalyxin and a proposed erythroblast niche. Blood, 2007-04-02;110(2):509-18. 2007-04-02 [PMID: 17403918] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: PODXL
• Uniprot:
  • Q9R0M4()